Evidence map›Paper›PMID 34160805›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

In Vitro Silencing of lncRNAs Using LNA GapmeRs.

Elisa Taiana, Vanessa Favasuli, Domenica Ronchetti, Eugenio Morelli, Pierfrancesco Tassone, Giuseppe Viglietto, Nikhil C Munshi, Antonino Neri, Nicola Amodio

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.4field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Elisa TaianaDepartment of Oncology and Hemato-oncology, University of Milan, Milan, Italy.
Vanessa FavasuliDepartment of Oncology and Hemato-oncology, University of Milan, Milan, Italy.
Domenica RonchettiDepartment of Oncology and Hemato-oncology, University of Milan, Milan, Italy.
Eugenio MorelliDepartment of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA.
Pierfrancesco TassoneDepartment of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Catanzaro, Italy.
Giuseppe VigliettoDepartment of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Catanzaro, Italy.
Nikhil C MunshiDepartment of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA.
Antonino NeriDepartment of Oncology and Hemato-oncology, University of Milan, Milan, Italy. antonino.neri@unimi.it.
Nicola AmodioDepartment of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, Catanzaro, Italy.
Magna Graecia University · ITFondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico · ITUniversity of Milan · ITDana-Farber Cancer Institute · USHarvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite substantial advancements have been achieved in the identification of long noncoding RNA (lncRNA) molecules, many challenges still remain into their functional characterization. Loss-of-function approaches are needed to study oncogenic lncRNAs, which appear more difficult to knock down by RNA interference as compared to mRNAs. In this chapter, we present a protocol based on the use of a novel class of antisense oligonucleotides, named locked nucleic acid (LNA) GapmeRs, to inhibit the oncogenic lncRNA NEAT1 in multiple myeloma cells. Overall, this approach holds many advantages, including its possible independence from delivery reagents as well as the capability to knock down lncRNAs even in hard-to-transfect suspension cells, like hematopoietic cells.

Indexed as

Gene Knockdown TechniquesGene SilencingOligonucleotidesBiomarkers, TumorCell Line, TumorElectroporationHumansRNA InterferenceRNA, Long NoncodingRNA, MessengerTransfectionBiomarkers, Tumorlocked nucleic acidNEAT1 long non-coding RNA, humanOligonucleotidesRNA, Long NoncodingRNA, MessengerASOElectroporationGymnosisLNA-GapmeRlncRNAMultiple myeloma cellsncRNA

Identifiers

PMID34160805
OpenAlexW3176280252

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.