ArticleThe Journal of cell biology2021
High-precision targeting workflow for volume electron microscopy.
Article in The Journal of cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 60 citations in OpenAlex.
- Diel remodeling and cellular integration of the nitroplast.bioRxiv : the preprint server for biology · 2026Article
- An Advanced Mobile Laboratory to enable field-based microbial ecology and cell biology across scales.bioRxiv : the preprint server for biology · 2026Article
- Microscopy Nodes: versatile 3D microscopy visualization with Blender.EMBO reports · 2026Article
- Cellular Hallmarks From Volume Electron Microscopy Reveal Developmental Progression of Plasmodium Ookinetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing.Scientific reports · 2025Article
- Article
- A workflow for semi-automated volume correlative light microscopy and transmission electron tomography.Journal of microscopy · 2025Article
- Mitochondrial ultrastructural pathology in diabetic cardiomyopathy: integrated analysis via scanning electron microscopy and 3D visualization imaging.Cardiovascular diabetology · 2025Article
- CELS-3D-Cutting edge light source for exciting fluorescence in microtome-based 3D microscopy and targeted correlative microscopy.Journal of anatomy · 2025Article
- Highlighting recent achievements to advance more effective cancer immunotherapy.Journal of experimental & clinical cancer research : CR · 2025Article
- A comprehensive overview of focused ion beam-scanning electron microscopy (FIB-SEM) applications for the evaluation of outer retina.Frontiers in cell and developmental biology · 2025Review
- Article
- Morphomics via next-generation electron microscopy.Journal of molecular cell biology · 2024Review
- Serial Block Face-Scanning Electron Microscopy as a Burgeoning Technology.Advanced biology · 2023Review
- Targeted volume correlative light and electron microscopy of an environmental marine microorganism.Journal of cell science · 2023Article
- Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP.Communications biology · 2023Article
- Light and electron microscopy continuum-resolution imaging of 3D cell cultures.Developmental cell · 2023Article
- Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences.Applied physics letters · 2023Article
- Light and focused ion beam microscopy workflow for resin-embedded tissues.Frontiers in cell and developmental biology · 2023Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
Cells are 3D objects. Therefore, volume EM (vEM) is often crucial for correct interpretation of ultrastructural data. Today, scanning EM (SEM) methods such as focused ion beam (FIB)-SEM are frequently used for vEM analyses. While they allow automated data acquisition, precise targeting of volumes of interest within a large sample remains challenging. Here, we provide a workflow to target FIB-SEM acquisition of fluorescently labeled cells or subcellular structures with micrometer precision. The strategy relies on fluorescence preservation during sample preparation and targeted trimming guided by confocal maps of the fluorescence signal in the resin block. Laser branding is used to create landmarks on the block surface to position the FIB-SEM acquisition. Using this method, we acquired volumes of specific single cells within large tissues such as 3D cultures of mouse mammary gland organoids, tracheal terminal cells in Drosophila melanogaster larvae, and ovarian follicular cells in adult Drosophila, discovering ultrastructural details that could not be appreciated before.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.