ArticleSTAR protocols2021
Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Preparation of Postmortem Human Formalin-Fixed Paraffin-Embedded Frontal Cortex Tissue for Profiling Pyramidal Neurons Via Digital Spatial Profiling.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Base editing rescues a hereditary motor neuron disease in mouse and patient-derived iPSC organoid models.Molecular therapy. Advances · 2026Article
- Plant-derived mitochondria mitigate aging-related neurodegeneration by reprogramming microglial mitochondrial energy metabolism.Translational neurodegeneration · 2026Article
- ACSA2 is not astrocyte-specific: implications for cell sorting strategies in the rodent brain.Frontiers in cellular neuroscience · 2026Article
- Early-life oxidative stress programs persistent NFκB activation and neuroinflammation in autism spectrum disorder.Frontiers in immunology · 2026Article
- Profiling lamina specific pyramidal neurons using postmortem human formalin fixed paraffin embedded frontal cortex tissue in combination with digital spatial profiling.Journal of neuroscience methods · 2025Article
- Inhibition of autophagy-lysosomal function exacerbates microglial and monocyte lipid metabolism reprograming and dysfunction after brain injury.bioRxiv : the preprint server for biology · 2025Article
- Astrocyte diversity and subtypes: aligning transcriptomics with multimodal perspectives.EMBO reports · 2025Review
- Perinatal hypoxia-mediated neurodevelopment abnormalities in congenital heart disease mouse model.Molecular medicine (Cambridge, Mass.) · 2025Article
- Applying single-cell and single-nucleus genomics to studies of cellular heterogeneity and cell fate transitions in the nervous system.Nature neuroscience · 2024Review
- T cell-mediated microglial activation triggers myelin pathology in a mouse model of amyloidosis.Nature neuroscience · 2024Article
- Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice.Nature communications · 2024Article
- Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury.Nature communications · 2023Article
- Myelin insulation as a risk factor for axonal degeneration in autoimmune demyelinating disease.Nature neuroscience · 2023Article
- Article
- Methanol fixation is the method of choice for droplet-based single-cell transcriptomics of neural cells.Communications biology · 2023Article
- Neuro-immune interactions at single-cell resolution in neurodevelopmental, infectious, and neurodegenerative diseases.Animal cells and systems · 2022Review
- Prenatal Progestin Exposure-Mediated Oxytocin Suppression Contributes to Social Deficits in Mouse Offspring.Frontiers in endocrinology · 2022Article
- Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue.Journal of visualized experiments : JoVE · 2021Article
- Brain-derived neurotrophic factor levels and oxidative stress in autism: evidence from children and a mouse model.Journal of psychiatry & neuroscience : JPNArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system's transcriptional responses during health and disease. However, dissociating brain cells is challenging and induces artificial transcriptional responses. Here, we describe an enzymatic dissociation method for mouse brain that prevents dissociation artifacts and lowers technical variations with standardized steps. We tested this protocol on microdissected brain tissue of 3-week- to 24-month-old mice and obtained high-quality scRNA-seq results. For complete details on the use and execution of this protocol, please refer to Safaiyan et al. (2021).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.