ArticleScientific reports2021
Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Examining hybridization-based LC-MS methodologies for the bioanalysis of siRNA analytes.Bioanalysis · 2025Article
- Iron Oxide Nanoparticles Modified with Galloylated DNA for Magnetically Enhanced DNA-Directed Assembly.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Nanopore-based amplicon sequencing for rapid detection and identification ofFrontiers in microbiology · 2025Article
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8 authors.
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Abstract
Detection of low abundance target DNA/RNA for clinical or research purposes is challenging because the target sequences can be hidden under a large background of human genomic or non-human metagenomic sequences. We describe a probe-based capture method to enrich for target sequences with DNA-clicked iron oxide nanoparticles. Our method was tested against commercial capture assays using streptavidin beads, on a set of probes derived from a common genotype of the hepatitis C virus. We showed that our method is more specific and sensitive, most likely due to the combination of an inert silica coating and a high density of DNA probes clicked to the nanoparticles. This facilitates target capture below the limits of detection for TaqMan qPCR, and we believe that this method has the potential to transform management of infectious diseases.
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