ArticleNature communications2021
Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- PAX3-SIX2 muscle stem cell heterogeneity drives muscle-specific repair dynamics diversification.Science advances · 2026Article
- Myotendinous Junction development and repair.Journal of orthopaedic translation · 2026Review
- Non-canonical muscle stem cells and other lessons from regenerative vertebrates.NPJ Regenerative medicine · 2026Review
- Article
- The contribution of skeletal muscle interstitial cells to myogenesis.Skeletal muscle · 2026Review
- Deciphering skeletal muscle development: cellular heterogeneity and molecular regulatory networks from single-cell and spatial transcriptomic perspectives.Frontiers in cell and developmental biology · 2026Review
- The repair capacity spectrum of human skeletal muscle injury from sports to surgical trauma settings.The Journal of physiology · 2025Review
- Dynamic transcriptional and epigenetic changes define postnatal tendon growth.PLoS genetics · 2025Article
- Differentially expressed fusogens specify myocyte states to drive myogenesis.Development (Cambridge, England) · 2025Article
- Muscle Stem Cell Microenvironment and Functions in Muscle Regeneration.Biomolecules · 2025Review
- Spatial transcriptomics in the adulteLife · 2025Article
- 3D-environment and muscle contraction regulate the heterogeneity of myonuclei.Skeletal muscle · 2024Article
- Single-cell transcriptomic analysis of flowering regulation and vernalization in Chinese cabbage shoot apex.Horticulture research · 2024Article
- The diagnostic value and associated molecular mechanism study for fibroblast-related mitochondrial genes on keloid.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
- Limb connective tissue is organized in a continuum of promiscuous fibroblast identities during development.iScience · 2024Article
- Exploring stem cell frontiers: definitions, challenges, and perspectives for regenerative medicine.Biology open · 2024Review
- Regeneration process of myotendinous junction injury induced by collagenase injection between Achilles tendon and soleus muscle in mice.Anatomical science international · 2024Article
- Effects of Myostatin on Nuclear Morphology at the Myotendinous Junction.International journal of molecular sciences · 2023Article
- Hox11-expressing interstitial cells contribute to adult skeletal muscle at homeostasis.Development (Cambridge, England) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 4 institutions in 3 countries.
Funding
Abstract
Positional information driving limb muscle patterning is contained in connective tissue fibroblasts but not in myogenic cells. Limb muscles originate from somites, while connective tissues originate from lateral plate mesoderm. With cell and genetic lineage tracing we challenge this model and identify an unexpected contribution of lateral plate-derived fibroblasts to the myogenic lineage, preferentially at the myotendinous junction. Analysis of single-cell RNA-sequencing data from whole limbs at successive developmental stages identifies a population displaying a dual muscle and connective tissue signature. BMP signalling is active in this dual population and at the tendon/muscle interface. In vivo and in vitro gain- and loss-of-function experiments show that BMP signalling regulates a fibroblast-to-myoblast conversion. These results suggest a scenario in which BMP signalling converts a subset of lateral plate mesoderm-derived cells to a myogenic fate in order to create a boundary of fibroblast-derived myonuclei at the myotendinous junction that controls limb muscle patterning.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.