ArticleGenes and environment : the official journal of the Japanese Environmental Mutagen Society2021
New homozygous gpt delta transgenic rat strain improves an efficiency of the in vivo mutagenicity assay.
Article in Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- Detection of in vivo mutagenicity in rat liver samples using error-corrected sequencing techniques.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2023Article
- Comparison of the frequencies of ENU-induced point mutations in male germ cells and inherited germline mutations in their offspring.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Mutagenicity of carcinogenic heterocyclic amines in Salmonella typhimurium YG strains and transgenic rodents including gpt delta.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundGene mutation assays in transgenic rodents are useful tools to investigate in vivo mutagenicity in a target tissue. Using a lambda EG10 transgene containing reporter genes, gpt delta transgenic mice and rats have been developed to detect point mutations and deletions. The transgene is integrated in the genome and can be rescued through an in vitro packaging reaction. However, the packaging efficiency is lower in gpt delta rats than in mice, because of the transgene in gpt delta rats being heterozygous and in low copy number. To improve the packaging efficiency, we herein describe a newly developed homozygous gpt delta rat strain.
resultsThe new gpt delta rat has a Wistar Hannover background and has been successfully maintained as homozygous for the transgene. The packaging efficiency in the liver was 4 to 8 times higher than that of existing heterozygous F344 gpt delta rats. The frequency of gpt point mutations significantly increased in the liver and bone marrow of N-nitroso-N-ethylurea (ENU)- and benzo[a]pyrene (BaP)-treated rats. Spi
conclusionsThe new homozygous gpt delta rat strain showed a higher packaging efficiency, and could be useful for in vivo gene mutation assays in rats.
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