ArticlePlant methods2021
Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis.
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- The Plant-Associated Flavobacterium: A Hidden Helper for Improving Plant Health.The plant pathology journal · 2024Review
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- Analytical methods for stable isotope labeling to elucidate rapid auxin kinetics in Arabidopsis thaliana.PloS one · 2024Article
- Can plant hormonomics be built on simple analysis? A review.Plant methods · 2023Review
- Pre-Harvest Salicylic Acid Application Affects Fruit Quality and Yield under Deficit Irrigation inPlants (Basel, Switzerland) · 2023Article
- Comparative Coexpression Analysis of Indole Synthase and Tryptophan Synthase A Reveals the Independent Production of Auxin via the Cytosolic Free Indole.Plants (Basel, Switzerland) · 2023Article
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Authors and funding
3 authors.
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Abstract
backgroundThe plant hormone auxin plays a central role in regulation of plant growth and response to environmental stimuli. Multiple pathways have been proposed for biosynthesis of indole-3-acetic acid (IAA), the primary auxin in a number of plant species. However, utilization of these different pathways under various environmental conditions and developmental time points remains largely unknown.
resultsMonitoring incorporation of stable isotopes from labeled precursors into proposed intermediates provides a method to trace pathway utilization and characterize new biosynthetic routes to auxin. These techniques can be aided by addition of chemical inhibitors to target specific steps or entire pathways of auxin synthesis.
conclusionsHere we describe techniques for pathway analysis in Arabidopsis thaliana seedlings using multiple stable isotope-labeled precursors and chemical inhibitors coupled with highly sensitive liquid chromatography-mass spectrometry (LC-MS) methods. These methods should prove to be useful to researchers studying routes of IAA biosynthesis in vivo in a variety of plant tissues.
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