Evidence map›Paper›PMID 34157727›Full record

ArticleDiving and hyperbaric medicine2021

Necrostatin-1 prolongs latency to convulsion in mice exposed to high oxygen partial pressure.

Zhen-Biao Guan, Yan-Yan Zhou, Yi Cen, Han-De Feng, Wen-Wu Liu, Hong-Jie Yi, Hui Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diving and hyperbaric medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Necrostatin-1: a promising compound for neurological disorders.Frontiers in cellular neuroscience · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Zhen-Biao GuanDepartment of Respiratory and Critical Illness, Changhai Hospital, the Naval Military Medical University, People's Liberation Army, Shanghai, 200433, China.
Yan-Yan ZhouDepartment of Orthopedic Surgery, Changzheng Hospital, the Naval Military Medical University, People's Liberation Army, Shanghai, 200003, China.
Yi CenDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Han-De FengDepartment of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Wen-Wu LiuDepartment of Diving and Hyperbaric Medicine, the Naval Military Medical University, People's Liberation Army, Shanghai, 200433, China.
Hong-Jie YiDepartment of Hyperbaric Oxygen, Changhai Hospital, the Naval Military Medical University, People's Liberation Army, Shanghai, 200433, China.
Hui ChenDepartment of Hyperbaric Oxygen, Changhai Hospital, the Naval Military Medical University, People's Liberation Army, Shanghai, 200433, China.
Tongji University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionExposure to very high oxygen partial pressure may cause central nervous system oxygen toxicity (CNS-OT). The role of necroptosis in the pathogenesis of CNS-OT is still unclear.

methodsIn experiment one, male C57BL/6 mice in the oxygen toxicity (OT) group (n = 5) and necrostatin-1 (Nec-1; a necroptosis inhibitor) (1.5 mg·kg-1, intraperitoneal) group (n = 5) were exposed to pure oxygen at 600 kPa, and the latency to tonic-clonic seizure was recorded. In experiment two, mice were divided into three groups: control group (n = 11), OT group (n = 12) and Nec-1 group (n = 12). Nec-1 was intraperitoneally administered 30 min before oxygen exposure. Mice in the OT group and Nec-1 group were exposed to pure oxygen at 400 kPa for 30 min, and then sacrificed; the brain was harvested for the assessment of inflammation, oxidative stress and necroptosis.

resultsExperiment one. Nec-1 pre-treatment significantly prolonged the latency to seizure (245 [SD 18] seconds in the OT group versus 336 (34) seconds in the Nec-1 group). Experiment two. Nec-1 pre-treatment markedly reduced inflammatory cytokines and inhibited cerebral necroptosis, but failed to significantly suppress cerebral oxidative stress.

conclusionsThese findings indicate necroptosis is involved in the pathogenesis of CNS-OT, and inhibition of necroptosis may prolong seizure latency, but the specific mechanisms should be investigated further.

Indexed as

OxygenSeizuresAnimalsApoptosisImidazolesIndolesMaleMiceMice, Inbred C57BLPartial PressureImidazolesIndolesnecrostatin-1OxygenAnimal modelCentral nervous systemInflammationNecroptosisOxidative stressOxygen toxicity

Identifiers

PMID34157727
PMCPMC8426125
OpenAlexW3173398404

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.