ArticleNucleic acids research2021
Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Benchmarking DNA barcode decoding strategies under high error rates.BMC bioinformatics · 2026Article
- Synthesis of highly complex phosphorothioate-modified oligonucleotides on microarrays.Scientific reports · 2026Article
- Comparison of state-of-the-art error-correction coding for sequence-based DNA data storage.Nature communications · 2026Article
- Fast barcode calling based onPNAS nexus · 2026Article
- DVOUG enables robust DNA sequence assembly and reconstruction with a dynamic, variable-order graph.Cell reports methods · 2025Article
- Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine OCommunications chemistry · 2025Article
- Insights into the utilisation of 1,2-propanediol and interactions with the cell envelope of Clostridium perfringens.Gut pathogens · 2025Article
- Recent progress in DNA data storage based on high-throughput DNA synthesis.Biomedical engineering letters · 2024Review
- Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ.Science advances · 2024Article
- Automated high-throughput DNA synthesis and assembly.Heliyon · 2024Review
- Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates.Analytical chemistry · 2024Article
- Reconstruction algorithms for DNA-storage systems.Scientific reports · 2024Article
- Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing.ACS synthetic biology · 2023Article
- A Canvas of Spatially Arranged DNA Strands that Can Produce 24-bit Color Depth.Journal of the American Chemical Society · 2023Article
- Dipodal Silanes Greatly Stabilize Glass Surface Functionalization for DNA Microarray Synthesis and High-Throughput Biological Assays.Analytical chemistry · 2023Article
- Enzymatic Synthesis of High-Density RNA Microarrays.Current protocols · 2023Article
- SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries.PloS one · 2023Article
- An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting.Nanoscale · 2022Article
- Fast trimer statistics facilitate accurate decoding of large random DNA barcode sets even at large sequencing error rates.PNAS nexus · 2022Article
- Robust data storage in DNA by de Bruijn graph-based de novo strand assembly.Nature communications · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 4 countries.
Funding
Abstract
Nucleic acid microarrays are the only tools that can supply very large oligonucleotide libraries, cornerstones of the nascent fields of de novo gene assembly and DNA data storage. Although the chemical synthesis of oligonucleotides is highly developed and robust, it is not error free, requiring the design of methods that can correct or compensate for errors, or select for high-fidelity oligomers. However, outside the realm of array manufacturers, little is known about the sources of errors and their extent. In this study, we look at the error rate of DNA libraries synthesized by photolithography and dissect the proportion of deletion, insertion and substitution errors. We find that the deletion rate is governed by the photolysis yield. We identify the most important substitution error and correlate it to phosphoramidite coupling. Besides synthetic failures originating from the coupling cycle, we uncover the role of imperfections and limitations related to optics, highlight the importance of absorbing UV light to avoid internal reflections and chart the dependence of error rate on both position on the array and position within individual oligonucleotides. Being able to precisely quantify all types of errors will allow for optimal choice of fabrication parameters and array design.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.