ArticleNature cell biology2021
Cell fate coordinates mechano-osmotic forces in intestinal crypt formation.
Article in Nature cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
80 citing papers in PubMed, 1 synthesis or guideline pooled it, 163 citations in OpenAlex.
- Pooled it
- Effect of oral hypotonic water and isotonic saline on physiological intestinalJournal of applied clinical medical physics · 2026Trial
- Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine.iScience · 2026Article
- Real-Time Monitoring of Mass and Mechanical Properties in Single Cells and Multicellular Spheroids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Fully Defined GelMA-Based Matrix Allows Fine Tuning of Tissue-Relevant Biomechanical and Biochemical Cues for Organoid Culture.Advanced healthcare materials · 2026Article
- A Single-organoid Workflow for quantitative Imaging classiFication and Tracking (SWIFT).Communications biology · 2026Article
- Article
- Biophysics of lumen morphogenesis.Development (Cambridge, England) · 2026Review
- Necking of epithelial tissues with cellular topological transition.PLoS computational biology · 2026Article
- New developments and applications of human organoids.Nature reviews. Molecular cell biology · 2026Review
- Stress-relaxing granular bioprinting materials enable complex and uniform organoid self-organization.Nature materials · 2026Article
- Advancing mechanobiology from single molecules to complex cellular systems.Nature nanotechnology · 2026Review
- Mechanochemical bistability as a principle of robust morphogenesis.Mechanobiology in medicine · 2026Article
- Hyaluronan Underlies the Emergence of Form, Fate, and Function in Human Cardioids.bioRxiv : the preprint server for biology · 2026Article
- Permeability-driven pressure and cell proliferation control lumen morphogenesis in pancreatic organoids.Nature cell biology · 2026Article
- Maternal Organ Growth: How the Adult Intestine Remodels During Pregnancy and Lactation.Development, growth & differentiation · 2026Review
- Article
- Convergent flow-mediated mesenchymal force drives embryonic foregut constriction and splitting.Nature communications · 2025Article
- The G1/S transition in mammalian stem cells in vivo is autonomously regulated by cell size.Nature communications · 2025Article
- Stress relaxing granular bioprinting materials enable complex and uniform organoid self-organization.bioRxiv : the preprint server for biology · 2025Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 4 countries.
Funding
Abstract
Intestinal organoids derived from single cells undergo complex crypt-villus patterning and morphogenesis. However, the nature and coordination of the underlying forces remains poorly characterized. Here, using light-sheet microscopy and large-scale imaging quantification, we demonstrate that crypt formation coincides with a stark reduction in lumen volume. We develop a 3D biophysical model to computationally screen different mechanical scenarios of crypt morphogenesis. Combining this with live-imaging data and multiple mechanical perturbations, we show that actomyosin-driven crypt apical contraction and villus basal tension work synergistically with lumen volume reduction to drive crypt morphogenesis, and demonstrate the existence of a critical point in differential tensions above which crypt morphology becomes robust to volume changes. Finally, we identified a sodium/glucose cotransporter that is specific to differentiated enterocytes that modulates lumen volume reduction through cell swelling in the villus region. Together, our study uncovers the cellular basis of how cell fate modulates osmotic and actomyosin forces to coordinate robust morphogenesis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.