ArticleProceedings of the National Academy of Sciences of the United States of America2021
CALM supports clathrin-coated vesicle completion upon membrane tension increase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Integrating Lateral Super-Resolution and Axial Progression Reveals Distinct Clathrin Pit Formation Pathways.Traffic (Copenhagen, Denmark) · 2026Article
- Growth-rate analysis of clathrin assembly: inferring endocytic kinetics in living cells and tissues.Frontiers in molecular biosciences · 2026Review
- Exploring the Spatial Limits of Extracellular Vesicles-Mediated Intercellular Communication.Journal of extracellular vesicles · 2025Article
- RAB23 facilitates clathrin-coated nascent vesicle formation at the plasma membrane and modulates cell signaling.Cellular and molecular life sciences : CMLS · 2025Article
- Risk of Alzheimer's disease in Down syndrome: Insights gained by multi-omics.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Review
- Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis.Cell death & disease · 2024Article
- Cell type-specific functions of Alzheimer's disease endocytic risk genes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.Journal of extracellular vesicles · 2024Article
- Protein condensates as flexible platforms for membrane traffic.Current opinion in cell biology · 2023Review
- Article
- Eukaryotic Clathrin Adapter Protein and Mediator of Cholesterol Homeostasis, PICALM, Affects Trafficking to the Chlamydial Inclusion.Molecular and cellular biology · 2023Article
- Biomechanical Role of Epsin in Influenza A Virus Entry.Membranes · 2022Article
- Insights into HIV uncoating from single-particle imaging techniques.Biophysical reviews · 2022Review
- Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension.Frontiers in molecular biosciences · 2022Article
- De novo endocytic clathrin coats develop curvature at early stages of their formation.Developmental cell · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The most represented components of clathrin-coated vesicles (CCVs) are clathrin triskelia and the adaptors clathrin assembly lymphoid myeloid leukemia protein (CALM) and the heterotetrameric complex AP2. Investigation of the dynamics of AP180-amino-terminal-homology (ANTH) recruitment during CCV formation has been hampered by CALM toxicity upon overexpression. We used knock-in gene editing to express a C-terminal-attached fluorescent version of CALM, while preserving its endogenous expression levels, and cutting-edge live-cell microscopy approaches to study CALM recruitment at forming CCVs. Our results demonstrate that CALM promotes vesicle completion upon membrane tension increase as a function of the amount of this adaptor present. Since the expression of adaptors, including CALM, differs among cells, our data support a model in which the efficiency of clathrin-mediated endocytosis is tissue specific and explain why CALM is essential during embryogenesis and red blood cell development.
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