ArticleJournal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy2021
Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis.
Article in Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Rapid monitoring of SARS-CoV-2 variants of concern through high-resolution melt analysis.Scientific reports · 2023Article
- A modified high-resolution melting-based assay (HRM) to identify the SARS-CoV-2 N501Y variant.Journal of virological methods · 2023Article
- A Rapid Screening Assay for Clarithromycin-Resistant Mycobacterium avium Complex Using Melting Curve Analysis with Nonfluorescent Labeled Probes.Microbiology spectrum · 2023Article
- Evaluation of an identification method for the SARS-CoV-2 Delta variant based on the amplification-refractory mutation system.Frontiers in cellular and infection microbiology · 2023Article
- Assessment of a mass balance equation for estimating community-level prevalence of COVID-19 using wastewater-based epidemiology in a mid-sized city.Scientific reports · 2022Article
- High-resolution melting analysis to discriminate between the SARS-CoV-2 Omicron variants BA.1 and BA.2.Biochemistry and biophysics reports · 2022Article
- Identification of SARS-CoV-2 Variants of Concern Using Amplicon Next-Generation Sequencing.Microbiology spectrum · 2022Article
- Discrimination of SARS-CoV-2 Omicron Sublineages BA.1 and BA.2 Using a High-Resolution Melting-Based Assay: a Pilot Study.Microbiology spectrum · 2022Article
- PCR-Based Screening Tests for SARS-CoV-2 Mutations: What Is the Best Way to Identify Variants?Clinical chemistry · 2022Article
- High-resolution melting analysis after nested PCR for the detection of SARS-CoV-2 spike protein G339D and D796Y variations.Biochemical and biophysical research communications · 2022Article
- A Rapid and Consistent Method to Identify Four SARS-CoV-2 Variants during the First Half of 2021 by RT-PCR.Vaccines · 2022Article
- Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform.Biosensors & bioelectronics · 2022Article
- Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting.Diagnostics (Basel, Switzerland) · 2021Article
- Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.PloS one · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus causing coronavirus disease 2019 (COVID-19), has been expanding globally since late 2019. SARS-CoV-2, an RNA virus, has a genome sequence that can easily undergo mutation. Several mutated SARS-CoV-2 strains, including those with higher infectivity than others, have been reported. To reduce SARS-CoV-2 transmission, it is crucial to trace its infection sources. Here, we developed a simple, easy-to-use genotyping method to identify SARS-CoV-2 variants using a high-resolution melting (HRM) analysis.
methodsWe investigated five mutation sites, A23403G, G25563T, G26144T, T28144C, and G28882A, which are known strain determinants according to GISAID clades (L, S, V, G, GH, and GR).
resultsWe first employed synthetic DNA fragments containing the five characteristic sites for HRM analysis. All sequences clearly differentiated wild-type from mutant viruses. We then confirmed that RNA fragments were suitable for HRM analysis following reverse transcription. Human saliva did not negatively affect the HRM analysis, which supports the absence of a matrix effect.
conclusionsOur results indicate that this HRM-based genotyping method can identify SARS-CoV-2 variants. This novel assay platform potentially paves the way for accurate and rapid identification of SARS-CoV-2 infection sources.
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