Evidence map›Paper›PMID 34154674›Full record

ArticleOne health outlook2021

Sero-molecular epidemiology of hepatitis E virus in pigs and human contacts in Ghana.

Richmond Yeboah, Augustina Angelina Sylverken, Michael Owusu, Philip El-Duah, Vitus Burimuah, Yaw Frimpong, Jones Lamptey, Isabella Eckerle, Benjamin Meyer, Christopher Antwi and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in One health outlook, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Richmond YeboahKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Augustina Angelina SylverkenKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Michael OwusuKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Philip El-DuahKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Vitus BurimuahKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Yaw FrimpongKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Jones LampteyKumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Isabella EckerleGeneva Centre for Emerging Viral Diseases, Geneva, Switzerland.
Benjamin MeyerInstitute of Virology, University of Bonn Medical Centre, Bonn, Germany.
Christopher AntwiDepartment of Animal Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Olivia AgbenyagaDepartment of Agroforestry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Raphael FolitseSchool of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Benjamin EmikpeDepartment of Pathobiology, School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Samuel Kingsley OppongDepartment of Wildlife and Range Management, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Yaw Adu-SarkodieDepartment of Clinical Microbiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Christian DrostenInstitute of Virology, Charite, Berlin, Germany. christian.drosten@charite.de.
Kwame Nkrumah University of Science and Technology · GHKumasi Centre for Collaborative Research in Tropical Medicine · GHCharité - Universitätsmedizin Berlin · DEUniversity of Bonn · DE

Funding

Deutsche Forschungsgemeinschaft DR 772/12-1
6 · The paper itself

Abstract

backgroundHepatitis E virus (HEV) is among the leading causes of viral hepatitis in most developing countries. Zoonotic acquisition of HEV genotype 3 from swine has come into focus more recently. Available studies on HEV in Ghana and other countries in the region do not provide enough information towards understanding the epidemiology of HEV in human and animal populations. Towards this end, we conducted a comparative cross-sectional study to determine the seroprevalence and risk factors associated with HEV exposure, both in swine and humans working on pig farms in typical local settings. The presence of viral RNA in human and swine samples was also evaluated, along with classification of viral sequences from HEV-positive samples.

methodsStructured questionnaires soliciting information on pigs reared, as well as socio-demographic information including age, sex and educational background of humans was collected. A total of 10 ml and 5 ml of whole blood was collected from pigs and human participants respectively. ELISA and real-time RT-PCR were performed on the sera for the qualitative detection of IgG antibodies to hepatitis E virus and viral RNA, respectively.

resultsFive hundred and forty-four (544) human participants including 264 swine contacts and 280 swine non-contacts were enrolled in the study. Although the proportion of HEV IgG antibodies was higher in contact groups (114; 54.3%) than non-contact groups (96; 45.7%), a multivariate analysis did not show any significant difference. No HEV RNA was detected in human samples. Similarly, 720 pigs were sampled from 18 farms located in five regions in Ghana. Twenty-three (23) of the pigs (3.2, 95%CI = 2.0-4.8) were positive for HEV RNA by real-time RT-PCR testing. Sequences obtained from HEV-positive samples were found to share high sequence identities with each other and clustered with other genotype 3 viruses indicating the existence of circulating zoonotic genotype 3 viruses on farms. Although we did not find evidence of pig to human transmission of HEV genotype 3, the presence of this genotype in pigs shows the potential for possible zoonotic transmission in African farm settings and buttresses the importance of active surveillance for the infection among at risk populations.

Indexed as

Infectious disease reservoirsLivestockOne healthViral hepatitisZoonoses

Identifiers

PMID34154674
PMCPMC8218416
OpenAlexW3176116779

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.