ArticlePLoS genetics2021
High-resolution genetic mapping reveals cis-regulatory and copy number variation in loci associated with cytochrome P450-mediated detoxification in a generalist arthropod pest.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Genomic analysis of population structure and target-site mutations of Tetranychus truncatus in China.Pest management science · 2026Article
- A genome sequence and efficient CRISPR/Cas9 gene editing tools for the solanaceous specialist pest Tetranychus evansi.Insect biochemistry and molecular biology · 2026Article
- Population genomics reveal multiple independent origins of pesticide resistance in the polyphagous pest, Tetranychus urticae.Communications biology · 2025Article
- Expression reduction and a variant of a P450 gene mediate chlorpyrifos resistance in Tetranychus urticae Koch.Journal of advanced research · 2025Article
- Over-expression and increased copy numbers of a cytochrome P450 and two UDP-glucuronosyltransferase genes in macrocyclic lactone resistant Psoroptes ovis of cattle.PLoS pathogens · 2025Article
- Chromosome-level genome assembly of the two-spotted spider mite Tetranychus urticae.Scientific data · 2024Article
- Genome-Wide Comparative Analysis of the Cytochrome P450 Monooxygenase Family in 19 Aphid Species and Their Expression Analysis in 4 Cereal Crop Aphids.International journal of molecular sciences · 2024Article
- A nuclear receptor HR96-related gene underlies large trans-driven differences in detoxification gene expression in a generalist herbivore.Nature communications · 2023Article
- Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest.Evolutionary applications · 2023Article
- Genome-Wide Identification and Expression Analysis of the Cytochrome P450 Gene Family inInternational journal of molecular sciences · 2023Article
- Multiple-P450 Gene Co-Up-Regulation in the Development of Permethrin Resistance in the House Fly,International journal of molecular sciences · 2023Article
- Trans-driven variation in expression is common among detoxification genes in the extreme generalist herbivore Tetranychus urticae.PLoS genetics · 2022Article
- A chromosome-level genome of Semiothisa cinerearia provides insights into its genome evolution and control.BMC genomics · 2022Article
- Utility and challenges of using whole-genome resequencing to detect emerging insect and mite resistance in agroecosystems.Evolutionary applications · 2022Review
- MAPK-mediated transcription factor GATAd contributes to Cry1Ac resistance in diamondback moth by reducing PxmALP expression.PLoS genetics · 2022Article
- Article
- Lab-to-Field Transition of RNA Spray Applications - How Far Are We?Frontiers in plant science · 2021Review
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8 authors.
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Abstract
Chemical control strategies are driving the evolution of pesticide resistance in pest populations. Understanding the genetic mechanisms of these evolutionary processes is of crucial importance to develop sustainable resistance management strategies. The acaricide pyflubumide is one of the most recently developed mitochondrial complex II inhibitors with a new mode of action that specifically targets spider mite pests. In this study, we characterize the molecular basis of pyflubumide resistance in a highly resistant population of the spider mite Tetranychus urticae. Classical genetic crosses indicated that pyflubumide resistance was incompletely recessive and controlled by more than one gene. To identify resistance loci, we crossed the resistant population to a highly susceptible T. urticae inbred strain and propagated resulting populations with and without pyflubumide exposure for multiple generations in an experimental evolution set-up. High-resolution genetic mapping by a bulked segregant analysis approach led to the identification of three quantitative trait loci (QTL) linked to pyflubumide resistance. Two QTLs were found on the first chromosome and centered on the cytochrome P450 CYP392A16 and a cluster of CYP392E6-8 genes. Comparative transcriptomics revealed a consistent overexpression of CYP392A16 and CYP392E8 in the experimental populations that were selected for pyflubumide resistance. We further corroborated the involvement of CYP392A16 in resistance by in vitro functional expression and metabolism studies. Collectively, these experiments uncovered that CYP392A16 N-demethylates the toxic carboxamide form of pyflubumide to a non-toxic compound. A third QTL coincided with cytochrome P450 reductase (CPR), a vital component of cytochrome P450 metabolism. We show here that the resistant population harbors three gene copies of CPR and that this copy number variation is associated with higher mRNA abundance. Together, we provide evidence for detoxification of pyflubumide by cytochrome P450s that is likely synergized by gene amplification of CPR.
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