Protocol for crystal structure determination of the antagonist-bound human cannabinoid receptor CB2.

Ling Shen, Xiaoting Li, Junlin Liu, Kaiwen Liu, Zhi-Jie Liu, Tian Hua

Open access · goldAbstract read

In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 45% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Endocannabinoid-Binding Receptors as Drug Targets.Methods in molecular biology (Clifton, N.J.) · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ling SheniHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Xiaoting LiiHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Junlin LiuiHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Kaiwen LiuiHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Zhi-Jie LiuiHuman Institute, ShanghaiTech University, Shanghai 201210, China.

Tian HuaiHuman Institute, ShanghaiTech University, Shanghai 201210, China.

ShanghaiTech University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human cannabinoid receptor CB2 plays an important role in the immune system and is an attractive therapeutic target for pain and for inflammatory and neurodegenerative diseases. However, the structural basis of CB2 agonist selectivity is still elusive. Here, we describe a detailed protocol for the determination of the crystal structure of antagonist AM10257-bound CB2. This methodology could be applied to the structural studies of CB2 with diverse antagonists and agonists or to other class A G-protein-coupled receptors. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).

Indexed as

Amino Acid SequenceAnimalsBaculoviridaeCannabinoid Receptor AntagonistsCrystallography, X-RayHumansInsectaProtein BindingProtein ConformationReceptor, Cannabinoid, CB2Cannabinoid Receptor AntagonistsReceptor, Cannabinoid, CB2Molecular BiologyProtein expression and purificationStructural BiologyX-ray Crystallography

Identifiers

PMID34151302

DOI10.1016/j.xpro.2021.100584

PMCPMC8192724

OpenAlexW3170673524

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.