Evidence map›Paper›PMID 34151292›Full record

ArticleSTAR protocols2021

A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity.

Chun Liu, Pedro Medina, Dilip Thomas, Ian Y Chen, Karim Sallam, Danish Sayed, Nazish Sayed

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chun LiuStanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
Pedro MedinaStanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
Dilip ThomasStanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
Ian Y ChenStanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
Karim SallamStanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.
Danish SayedDepartment of Cell Biology and Molecular Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103, USA.
Nazish SayedStanford Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA.

Funding

Modeling Endothelial Dysfunction in LMNA-related Dilated CardiomyopathyK01HL135455 · NHLBI · STANFORD UNIVERSITY · PI SAYED, NAZISH · 2017 to 2021
$828k
NHLBI NIH HHS K01 HL135455
6 · The paper itself

Abstract

Endothelial cells (ECs) have emerged as key pathogenic players in cardiac disease due to their proximity with cardiomyocytes. Induced pluripotent stem cells (iPSCs) have been employed to generate ECs. However, it may be more clinically relevant to transdifferentiate fibroblasts into ECs directly without introducing pluripotent or virally driven transcription factors. Here, we present a protocol that describes the direct conversion of human cardiac fibroblasts into ECs by leveraging the innate immune system. Our protocol produces bona fide human ECs with 95%-98% purity by first passage. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Sayed et al. (2015).

Indexed as

Cell TransdifferentiationImmunity, InnateCulture MediaEndothelial CellsFibroblastsHumansMyocytes, CardiacTranscription FactorsCulture MediaTranscription FactorsCell cultureCell DifferentiationFlow Cytometry/Mass CytometryMolecular BiologyStem Cells

Identifiers

PMID34151292
PMCPMC8190482

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.