ArticleiScience2021
Rapid methods for the evaluation of fluorescent reporters in tissue clearing and the segmentation of large vascular structures.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Visualizing C. difficile During Murine Infection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optical tissue clearing and 3D imaging of intact primate testicular tissue: a novel technology development.PloS one · 2025Article
- Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.Chemical & biomedical imaging · 2024Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy.Scientific reports · 2024Article
- Therapy-induced modulation of tumor vasculature and oxygenation in a murine glioblastoma model quantified by deep learning-based feature extraction.Scientific reports · 2024Article
- Tissue clearing method in visualization of cancer progression and metastasis.Upsala journal of medical sciences · 2024Review
- 3D Optical Reconstruction of the Nervous System of the Whole-Body Marine Invertebrates.Chemical & biomedical imaging · 2023Article
- Effect of methanol fixation on single-cell RNA sequencing of the murine dentate gyrus.Frontiers in molecular neuroscience · 2023Article
- An analysis modality for vascular structures combining tissue-clearing technology and topological data analysis.Nature communications · 2022Article
- Tissue clearing may alter emission and absorption properties of common fluorophores.Scientific reports · 2022Article
- Multimodal Imaging of Target Detection Algorithm under Artificial Intelligence in the Diagnosis of Early Breast Cancer.Journal of healthcare engineering · 2022Article
- Drug-Induced Nephrotoxicity Assessment in 3D Cellular Models.Micromachines · 2021Review
- Scalable robust graph and feature extraction for arbitrary vessel networks in large volumetric datasets.BMC bioinformatics · 2021Article
- Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?International journal of molecular sciences · 2021Review
- Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities.PloS one · 2021Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light sheet fluorescence microscopy (LSFM) of large tissue samples does not require mechanical sectioning and allows efficient visualization of spatially complex or rare structures. Therefore, LSFM has become invaluable in developmental and biomedical research. Because sample size may limit whole-mount staining, LSFM benefits from transgenic reporter organisms expressing fluorescent proteins (FPs) and, however, requires optical clearing and computational data visualization and analysis. The former often interferes with FPs, while the latter requires massive computing resources. Here, we describe 3D-polymerized cell dispersions, a rapid and straightforward method, based on recombinant FP expression in freely selectable tester cells, to evaluate and compare fluorescence retention in different tissue-clearing protocols. For the analysis of large LSFM data, which usually requires huge computing resources, we introduce a refined, interactive, hierarchical random walker approach that is capable of efficient segmentation of the vasculature in data sets even on a consumer grade PC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.