Evidence map›Paper›PMID 34151220›Full record

ArticleACS applied bio materials2020

Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay.

Brian J Nablo, Jung-Joon Ahn, Kiran Bhadriraju, Jong Muk Lee, Darwin R Reyes

Abstract read
In one paragraph

Article in ACS applied bio materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Brian J NabloSOL Inc., H business Park, 26, Beobwon-ro 9-gil, SongPa-gu, Seoul, Korea.
Jung-Joon AhnSOL Inc., H business Park, 26, Beobwon-ro 9-gil, SongPa-gu, Seoul, Korea.
Kiran BhadrirajuPhysical Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg, MD, United States.
Jong Muk LeeSOL Inc., H business Park, 26, Beobwon-ro 9-gil, SongPa-gu, Seoul, Korea.
Darwin R ReyesPhysical Measurement Laboratory, National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg, MD, United States.ORCID 0000-0002-2986-0966

Funding

Intramural NIST DOC 9999-NIST
6 · The paper itself

Abstract

Neutral red is a low-cost supravital stain for determining cell viability. The standard protocol relies on a destructive extraction process to release the accumulated dye for endpoint spectrophotometric quantification. We report a non-destructive, live-cell quantification of neutral red uptake using a compact lens-free system. Two light sources indentify the cell perimeter and quantify neutral red uptake. The quantification occurs during staining, thus eliminating the destructive extraction process and reducing assay time. Our system enables live quantification for continuous high-throughput screening of cell viability within confined spaces such as incubators.

Indexed as

3-D Cell CultureCell ViabilityHepG2Lens-free ImagingNeutral RedQuantitative Imaging

Identifiers

PMID34151220
PMCPMC8209770

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.