Evidence map›Paper›PMID 34151084›Full record

ArticleACS omega2021

Theoretical Design, Synthesis, and In Vitro Neurobiological Applications of a Highly Efficient Two-Photon Caged GABA Validated on an Epileptic Case.

Balázs Chiovini, Dénes Pálfi, Myrtill Majoros, Gábor Juhász, Gergely Szalay, Gergely Katona, Milán Szőri, Orsolya Frigyesi, Csilla Lukácsné Haveland, Gábor Szabó and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 6 institutions in 2 countries.

Balázs ChioviniThe Faculty of Information Technology, Pázmány Péter Catholic University, 50 Práter str., H-1083 Budapest, Hungary.
Dénes PálfiThe Faculty of Information Technology, Pázmány Péter Catholic University, 50 Práter str., H-1083 Budapest, Hungary.
Myrtill MajorosThe Faculty of Information Technology, Pázmány Péter Catholic University, 50 Práter str., H-1083 Budapest, Hungary.
Gábor JuhászThe Faculty of Information Technology, Pázmány Péter Catholic University, 50 Práter str., H-1083 Budapest, Hungary.
Gergely SzalayLaboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, 43 Szigony str., H-1083 Budapest, Hungary.
Gergely KatonaLaboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, 43 Szigony str., H-1083 Budapest, Hungary.
Milán SzőriInstitute of Chemistry, Faculty of Materials Science and Engineering, University of Miskolc, H-3515 Miskolc, Hungary.ORCID https://orcid.org/0000-0003-4895-0999
Orsolya FrigyesiChemistry Department, Femtonics Limited, Tűzoltó str. 59, H-1094 Budapest, Hungary.
Csilla Lukácsné HavelandChemistry Department, Femtonics Limited, Tűzoltó str. 59, H-1094 Budapest, Hungary.
Gábor SzabóTransgenic Facility, Institute of Experimental Medicine, 43 Szigony str., H-1083 Budapest, Hungary.
Ferenc ErdélyiTransgenic Facility, Institute of Experimental Medicine, 43 Szigony str., H-1083 Budapest, Hungary.
Zoltán MátéTransgenic Facility, Institute of Experimental Medicine, 43 Szigony str., H-1083 Budapest, Hungary.
Zoltán SzadaiThe Faculty of Information Technology, Pázmány Péter Catholic University, 50 Práter str., H-1083 Budapest, Hungary.
Miklós MadarászLaboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, 43 Szigony str., H-1083 Budapest, Hungary.ORCID https://orcid.org/0000-0001-7057-303X
Miklós DékányGedeon Richter Plc, Gyömrői str. 19-21, H-1103 Budapest, Hungary.
Imre G CsizmadiaDepartment of Chemistry, University of Toronto, 80 St. George Street, M5S 3H6 Toronto, Ontario, Canada.
Ervin KovácsChemistry Department, Femtonics Limited, Tűzoltó str. 59, H-1094 Budapest, Hungary.ORCID https://orcid.org/0000-0002-3939-6925
Balázs RózsaThe Faculty of Information Technology, Pázmány Péter Catholic University, 50 Práter str., H-1083 Budapest, Hungary.
Zoltán MucsiInstitute of Chemistry, Faculty of Materials Science and Engineering, University of Miskolc, H-3515 Miskolc, Hungary.ORCID https://orcid.org/0000-0003-3224-8847
HUN-REN Institute of Experimental Medicine · HUPázmány Péter Catholic University · HUUniversity of Miskolc · HUGedeon Richter (Hungary) · HUInstitute of Materials and Environmental Chemistry · HUUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this paper, we present an additional, new cage-GABA compound, called 4-amino-1-(4'-dimethylaminoisopropoxy-5',7'-dinitro-2',3'-dihydro-indol-1-yl)-1-oxobutane-γ-aminobutyric acid (iDMPO-DNI-GABA), and currently, this compound is the only photoreagent, which can be applied for GABA uncaging without experimental compromises. By a systematic theoretical design and successful synthesis of several compounds, the best reagent exhibits a high two-photon efficiency within the 700-760 nm range with excellent pharmacological behavior, which proved to be suitable for a complex epileptic study. Quantum chemical design showed that the optimal length of the cationic side chain enhances the two-photon absorption by 1 order of magnitude due to the cooperating internal hydrogen bonding to the extra nitro group on the core. This feature increased solubility while suppressing membrane permeability. The efficiency was demonstrated in a systematic, wide range of in vitro single-cell neurophysiological experiments by electrophysiological as well as calcium imaging techniques. Scalable inhibitory ion currents were elicited by iDMPO-DNI-GABA with appropriate spatial-temporal precision, blocking both spontaneous and evoked cell activity with excellent efficiency. Additionally, to demonstrate its applicability in a real neurobiological study, we could smoothly and selectively modulate neuronal activities during artificial epileptic rhythms first time in a neural network of GCaMP6f transgenic mouse brain slices.

Identifiers

PMID34151084
PMCPMC8210458
OpenAlexW3169955951

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.