ArticleFrontiers in veterinary science2021
Development of a Real-Time Quantitative RT-PCR Assay for Detection of Bovine Rhinitis B Virus.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Evidence-based recommendations for shower-out practices: assessing virus removal from hair using a surrogate non-enveloped virus.Microbiology spectrum · 2026Article
- Development of a Conventional Reverse Transcription-PCR System for Broad Detection of Bovine Rhinitis A Virus.Veterinary medicine and science · 2025Article
- Leaderless foot-and-mouth disease virus serotype O did not cause clinical disease and failed to establish a persistent infection in cattle.Emerging microbes & infections · 2024Article
- Discovery and characterization of BRBV-sheep virus in nasal swabs from domestic sheep in China.Frontiers in cellular and infection microbiology · 2024Article
- Detection and Genomic Characterization of Bovine Rhinitis Virus in China.Animals : an open access journal from MDPI · 2023Article
- Meta-Analysis of qPCR for Bovine Respiratory Disease Based on MIQE Guidelines.Frontiers in molecular biosciences · 2022Article
- Genome Characterization and Phylogenetic Analysis of the First Bovine Rhinitis B Virus Isolate in China.Frontiers in veterinary science · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine rhinitis B virus (BRBV) has been frequently identified in cattle diagnosed with bovine respiratory disease complex (BRDC) in recent years, suggesting its potential contribution to BRDC. The goal of this study was to develop a TaqMan-based real-time quantitative RT-PCR assay for efficient BRBV detection. A pair of primers and a probe were designed based on the 3D gene of the BRBV genome. The assay was specific for BRBV and able to exclude bovine rhinitis A virus, foot-and-mouth disease virus and Senecavirus A. The limit of detection of the assay was 4.46 copies per reaction. A standard curve was plotted, with a coefficient of determination of 0.999 in the concentration range of 10
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Registered trials
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