ArticleFrontiers in medicine2021
Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds.
Article in Frontiers in medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Prevalence of wound infections and related antimicrobial resistance in Goma, Democratic Republic of the Congo: a multicenter cross-sectional study.BMC infectious diseases · 2026Article
- Article
- Distribution and Antibiotic Resistance Characteristics of Pathogenic Bacteria in Chronic Wound Infections: A Five-Year Retrospective Study at a Tertiary Hospital.Infection and drug resistance · 2026Article
- Assessment of Microbiome-Based Pathogen Detection Using Illumina Short-Read and Nanopore Long-Read Sequencing in 144 Patients Undergoing Bronchoalveolar Lavage in a University Hospital in Germany.International journal of molecular sciences · 2025Article
- Insights into ecology, pathogenesis, and biofilm formation ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- Bedside inoculation accelerates detection and improves microbiological diagnosis efficiency compared to delayed inoculation.American journal of translational research · 2025Article
- Metagenomic analysis reveals the community composition of the microbiome in different segments of the digestive tract in donkeys and cows: implications for microbiome research.BMC microbiology · 2024Article
- Deciphering microbiome and fungi-bacteria interactions in chronic wound infections using metagenomic sequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Bacteriophage Therapy on an In Vitro Wound Model and Synergistic Effects in Combination with Beta-Lactam Antibiotics.Antibiotics (Basel, Switzerland) · 2024Article
- Article
- Carbonate precipitation and phosphate trapping by microbialite isolates from an alkaline insular lake (Bagno dell'Acqua, Pantelleria Island, Italy).Frontiers in microbiology · 2024Article
- Relative Abundance and Detection ofMicroorganisms · 2023Review
- Colonizing microbiota is associated with clinical outcomes in diabetic wound healing.Advanced drug delivery reviews · 2023Review
- The Skin Microbiome: Current Landscape and Future Opportunities.International journal of molecular sciences · 2023Review
- Effectiveness of Epidermal Growth Factor Loaded Carboxymethylcellulose (EGF-CMC) Hydrogel in Biofilm Formation in Wounds of Diabetic Patients: A Randomized Clinical Trial.Gels (Basel, Switzerland) · 2023Article
- The Antimicrobial Effect of Various Single-Strain and Multi-Strain Probiotics, Dietary Supplements or Other Beneficial Microbes against Common Clinical Wound Pathogens.Microorganisms · 2022Article
- Wound swabs versus biopsies to detect methicillin resistant Staphylococcus aureus in experimental equine wounds.Veterinary surgery : VS · 2022Article
- Bacterial Interactions in the Context of Chronic Wound Biofilm: A Review.Microorganisms · 2022Review
- Comparison Between Cultivation and Sequencing Based Approaches for Microbiota Analysis in Swabs and Biopsies of Chronic Wounds.Frontiers in medicine · 2021Article
- What is slough? Defining the proteomic and microbial composition of slough and its implications for wound healing.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyArticle
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chronic wounds are a prominent health concern affecting 0.2% of individuals in the Western population. Microbial colonization and the consequent infection contribute significantly to the healing process. We have compared two methods, cultivation and 16S amplicon sequencing (16S-AS), for the characterization of bacterial populations in both swabs and biopsy tissues obtained from 45 chronic wounds. Using cultivation approach, we detected a total of 39 bacterial species, on average 2.89 per sample (SD = 1.93), compared to 5.9 (SD = 7.1) operational taxonomic units per sample obtained with 16S-AS. The concordance in detected bacteria between swab and biopsy specimens obtained from the same CWs was greater when using cultivation (58.4%) as compared to 16S-AS (25%). In the entire group of 45 biopsy samples concordance in detected bacterial genera between 16S-AS and cultivation-based approach was 36.4% and in swab samples 28.7%. Sequencing proved advantageous in comparison to the cultivation mainly in case of highly diverse microbial communities, where we could additionally detect numerous obligate and facultative anaerobic bacteria from genera
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