ArticleFrontiers in bioengineering and biotechnology2021
Engineering of Chinese Hamster Ovary Cells With NDPK-A to Enhance DNA Nuclear Delivery Combined With EBNA1 Plasmid Maintenance Gives Improved Exogenous Transient Reporter, mAb and SARS-CoV-2 Spike Protein Expression.
Article in Frontiers in bioengineering and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.
- Pooled it
- Article
- Mapping cellular processes that determine delivery of plasmid DNA to the nucleus: application in Chinese hamster ovary and human embryonic kidney cells to enhance protein production.Frontiers in bioengineering and biotechnology · 2025Article
- Improvement strategies for transient gene expression in mammalian cells.Applied microbiology and biotechnology · 2024Review
- Resource-aware construct design in mammalian cells.Nature communications · 2023Article
- A proline metabolism selection system and its application to the engineering of lipid biosynthesis in Chinese hamster ovary cells.Metabolic engineering communications · 2021Article
- Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO Cells.Frontiers in bioengineering and biotechnology · 2021Article
- Enhancing recombinant antibody yield in Chinese hamster ovary cells.Tzu chi medical journalReview
- Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient gene expression (TGE) in mammalian cells is a method of rapidly generating recombinant protein material for initial characterisation studies that does not require time-consuming processes associated with stable cell line construction. High TGE yields are heavily dependent on efficient delivery of plasmid DNA across both the plasma and nuclear membranes. Here, we harness the protein nucleoside diphosphate kinase (NDPK-A) that contains a nuclear localisation signal (NLS) to enhance DNA delivery into the nucleus of CHO cells. We show that co-expression of NDPK-A during transient expression results in improved transfection efficiency in CHO cells, presumably due to enhanced transportation of plasmid DNA into the nucleus via the nuclear pore complex. Furthermore, introduction of the Epstein Barr Nuclear Antigen-1 (EBNA-1), a protein that is capable of inducing extrachromosomal maintenance, when coupled with complementary
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Registered trials
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