Evidence map›Paper›PMID 34149858›Full record

ArticleIranian journal of veterinary research2021

P Xu, H Fotina, T Fotina, S Wang

Open access · greenAbstract read
In one paragraph

Article in Iranian journal of veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

P XuDepartment of Veterinary Expertise, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, 453003 Xinxiang, China.
H FotinaDepartment of Veterinary Expertise, Microbiology, Zoohygiene and Safety and Quality of Animals' Products, Faculty of Veterinary Medicine, Sumy National Agrarian University, 40021 Sumy, Ukraine.
T FotinaDepartment of Veterinary Expertise, Microbiology, Zoohygiene and Safety and Quality of Animals' Products, Faculty of Veterinary Medicine, Sumy National Agrarian University, 40021 Sumy, Ukraine.
S WangDepartment of Veterinary Expertise, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, 453003 Xinxiang, China.
Sumy National Agrarian University · UAHenan Institute of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease.

aimsThis study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from the milk of Ukraine Holstein dairy cows in order to develop a general

methodsThe milk from a three-year-old lactating dairy cow was used as a source of the epithelial cell, characteristics of BMECs were examined using real time cell assay (RTCA), immunocytochemistry (ICC), reverse transcription-polymerase chain reaction (RT-PCR), and Western blot (WB).

resultsThe results showed that BMECs can be recovered from milk, grown in culture, and exhibit the characteristic cobblestone morphology of epithelial cells.

conclusionThe established BMECs retained MEC characteristics and secreted β-caseins even when grew on plastic substratum. Thus, the established cell line had normal morphology, growth characteristics, as well as secretory characteristics, and it could be considered as a model system and useful tool for understanding the biology of dairy cow mammary glands.

Indexed as

Bovine mammary epithelial cellCobblestone morphologyMammary diseaseUkraine Holstein dairy cowβ-casein

Identifiers

PMID34149858
PMCPMC8195298
OpenAlexW3173596773

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.