Evidence map›Paper›PMID 34149662›Full record

ArticleFrontiers in microbiology2021

The Use of Ribosomal RNA as a Microbial Source Tracking Target Highlights the Assay Host-Specificity Requirement in Water Quality Assessments.

Annastiina Rytkönen, Ananda Tiwari, Anna-Maria Hokajärvi, Sari Uusheimo, Asko Vepsäläinen, Tiina Tulonen, Tarja Pitkänen

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Climate Change Impacts on Microbiota in Beach Sand and Water: Looking Ahead.International journal of environmental research and public health · 2022
    Review
  7. Review
  8. Bathing Water Quality Monitoring Practices in Europe and the United States.International journal of environmental research and public health · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Annastiina RytkönenExpert Microbiology Unit, Finnish Institute for Health and Welfare, Kuopio, Finland.
Ananda TiwariExpert Microbiology Unit, Finnish Institute for Health and Welfare, Kuopio, Finland.
Anna-Maria HokajärviExpert Microbiology Unit, Finnish Institute for Health and Welfare, Kuopio, Finland.
Sari UusheimoLammi Biological Station, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland.
Asko VepsäläinenEnvironmental Health Unit, Finnish Institute for Health and Welfare, Kuopio, Finland.
Tiina TulonenLammi Biological Station, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland.
Tarja PitkänenExpert Microbiology Unit, Finnish Institute for Health and Welfare, Kuopio, Finland.
Finnish Institute for Health and Welfare · FIUniversity of Helsinki · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

For microbial source tracking (MST), the 16S ribosomal RNA genes (rDNA) of host-specific bacteria and mitochondrial DNA (mtDNA) of animal species, known to cause fecal contamination of water, have been commonly used as molecular targets. However, low levels of contamination might remain undetected by using these DNA-based qPCR assays. The high copy numbers of ribosomal RNA (rRNA) could offer a solution for such applications of MST. This study compared the performance of eight MST assays: GenBac3 (general

Indexed as

fecal contaminationfield validationmicrobial source trackingperformance analysisribosomal RNART-qPCRsurface water

Identifiers

PMID34149662
PMCPMC8206488
OpenAlexW3169426362

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.