ArticleCytotechnology2021
Violacein improves recombinant IgG production by controlling the cell cycle of Chinese hamster ovary cells.
Article in Cytotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- Production, downstream, stability, bioactivities, and applications studies of violacein and related compounds.World journal of microbiology & biotechnology · 2025Review
- Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains.Bioprocess and biosystems engineering · 2024Article
- Effects and mechanism of small molecule additives on recombinant protein in CHO cells.Applied microbiology and biotechnology · 2023Review
- Effects and mechanisms of animal-free hydrolysates on recombination protein yields in CHO cells.Applied microbiology and biotechnology · 2022Review
- A Blue-Purple Pigment-Producing Bacterium Isolated from the Vezelka River in the City of Belgorod.Microorganisms · 2021Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chinese hamster ovary (CHO) cells are used as host cells for industrial monoclonal antibody (mAb) production. Cell cycle control is an effective approach to increase mAb production in the cell culture. Violacein, a purple-colored pigment produced by microorganisms, has diverse bioactive properties and has been proposed for various industrial applications. In this study, we evaluated the potency of violacein for cell cycle control and improvement of recombinant immunoglobulin G (IgG) production in CHO cells. Compared with the control, 0.9 μM violacein in a 14-day fed-batch culture increased the maximum IgG concentration by 37.6% via increasing the specific production rate and cell longevity. Cell cycle analysis showed that violacein induced G1 and G2/M phase arrest. However, the G1 arrest was observed only on day 1, while G2/M arrest lasted more than 3 days, suggesting that G2/M arrest mediated the violacein-induced enhanced IgG production. Moreover, in line with the increased protein expression, the expression levels of IgG mRNA and nutrient metabolic rates were also increased.
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Registered trials
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