Evidence map›Paper›PMID 34148878›Full record

ArticleJournal of biosciences2021

Relationship of intracellular proteolysis with CAP1 and cofilin1 in non-small-cell lung cancer.

E S Kolegova, G V Kakurina, E E Shashova, N V Yunusova, L V Spirina, E A Sidenko, D N Kostromitskiy, A Yu Dobrodeev, I V Kondakova

Abstract read
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In one paragraph

Article in Journal of biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

E S KolegovaTomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russian Federation 634050.
G V Kakurina
E E Shashova
N V Yunusova
L V Spirina
E A Sidenko
D N Kostromitskiy
A Yu Dobrodeev
I V Kondakova
Russian Academy of Sciences · RUTomsk Cancer Research Institute · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The main cause of death in non-small-cell lung cancer (NSCLC) is tumor progression, in which metastasis and invasion play an important role. The metastatic cascade is marked by a change in morphological, biological, biochemical and functional characteristics, including the acquisition of cellular mobility. The migration activity of tumor cells determines the work of actin-binding proteins that cause their functional partners CAP1 and cofilin. Of interest is the study of the regulation of working tandem CAP1/cofilin in NSCLC. The mechanism that regulates the level of proteins in cells is proteolysis, carried out by proteasomes and calpains. Therefore, the aim of this study was to estimate the expression of CAP1/CFL1 mRNA and their protein level in NSCLC tissues, and to analyze the possible mechanisms of their regulation by the proteasome and calpain systems. Samples of NSCLC and histological unchanged lung tissue were used (n = 42). The CAP1 and CFL1 mRNA expressions were determined by real-time PCR, the contents of proteins encoded by them were determined by Western blotting, and the activity of proteasomes and calpains by the fluorimetric method. There was an increase in the expression of mRNA and protein levels of CAP1 and cofilin in the tumor tissue compared with the unchanged lung tissue. The expression of mRNA and the level of CAP1 in tumor tissue increased during growth of the primary tumor. The cofilin level in the tumor tissue decreases against the background of increased expression of its mRNA. At the same time, during tumor growth, the activity of proteasomes and calpains increased. A negative regression relationships between the activity of proteasomes and the levels of CAP1 and cofilin, as well as the activity of calpains and the level of cofilin, were found. It can be assumed that proteasomes and calpains are involved in the degradation of CAP1 and cofilin. The data obtained suggest the importance of CAP1, cofilin and proteolytic systems in the tumor transformation and lymphogenous metastasis.

Indexed as

CalpainCarcinoma, Non-Small-Cell LungCell Cycle ProteinsCell MovementCofilin 1Cytoskeletal ProteinsFemaleGene Expression Regulation, NeoplasticHumansLung NeoplasmsLymphatic MetastasisMaleMiddle AgedProteolysisRNA, MessengerSignal TransductionCalpainCAP1 protein, humanCell Cycle ProteinsCFL1 protein, humanCofilin 1Cytoskeletal ProteinsRNA, Messenger

Identifiers

PMID34148878
OpenAlexW3166020892

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.