Evidence map›Paper›PMID 34147472›Full record

ArticleDevelopmental biology2021

Microtubule organization of vertebrate sensory neurons in vivo.

Matthew Shorey, Kavitha Rao, Michelle C Stone, Floyd J Mattie, Alvaro Sagasti, Melissa M Rolls

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Journal of medical genetics · 2025
    Article
  4. Ultrastructure of dorsal root ganglia.Cell and tissue research · 2023
    Review
  5. The MAP3Ks DLK and LZK Direct Diverse Responses to Axon Damage in Zebrafish Peripheral Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022
    Article
  6. Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Matthew ShoreyDepartment of Biochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
Kavitha RaoDepartment of Biochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
Michelle C StoneDepartment of Biochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
Floyd J MattieDepartment of Biochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
Alvaro SagastiMolecular, Cell and Developmental Biology Department and Molecular Biology Institute, University of California, Los Angeles, CA, 90095, USA.
Melissa M RollsDepartment of Biochemistry and Molecular Biology and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA. Electronic address: mur22@psu.edu.
Pennsylvania State University · USUniversity of California, Los Angeles · US

Funding

Using Drosophila Neurons to Identify Mechanisms that Control Microtubule PolarityR01GM085115 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI Melissa Rolls · 2010 to 2026
$5.1M
Do somatosensory endings use axonal or dendritic regeneration pathways?R21NS090027 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROLLS, MELISSA, SAGASTI, ALVARO · 2014 to 2015
$416k
NIGMS NIH HHS R01 GM085115NINDS NIH HHS R21 NS090027
6 · The paper itself

Abstract

Dorsal root ganglion (DRG) neurons are the predominant cell type that innervates the vertebrate skin. They are typically described as pseudounipolar cells that have central and peripheral axons branching from a single root exiting the cell body. The peripheral axon travels within a nerve to the skin, where free sensory endings can emerge and branch into an arbor that receives and integrates information. In some immature vertebrates, DRG neurons are preceded by Rohon-Beard (RB) neurons. While the sensory endings of RB and DRG neurons function like dendrites, we use live imaging in zebrafish to show that they have axonal plus-end-out microtubule polarity at all stages of maturity. Moreover, we show both cell types have central and peripheral axons with plus-end-out polarity. Surprisingly, in DRG neurons these emerge separately from the cell body, and most cells never acquire the signature pseudounipolar morphology. Like another recently characterized cell type that has multiple plus-end-out neurites, ganglion cells in Nematostella, RB and DRG neurons maintain a somatic microtubule organizing center even when mature. In summary, we characterize key cellular and subcellular features of vertebrate sensory neurons as a foundation for understanding their function and maintenance.

Indexed as

AnimalsAnimals, Genetically ModifiedAxonsCell BodyCell PolarityDendritesDrosophilaGanglia, SpinalMicrotubule-Organizing CenterMicrotubulesSea AnemonesSensory Receptor CellsSkinZebrafishDorsal root ganglion cellMicrotubule polarityNeuronal polarity

Identifiers

PMID34147472
PMCPMC8364508
OpenAlexW3176325998

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.