ArticleVirus research2021
A novel RdRp-based colorimetric RT-LAMP assay for rapid and sensitive detection of SARS-CoV-2 in clinical and sewage samples from Pakistan.
Article in Virus research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Development of RT-LAMP assay for variants of HCV in Pakistan.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Rapid identification of bacterial select agents using loop-mediated isothermal amplification.BMC infectious diseases · 2025Article
- Enhanced Specificity in Colorimetric LAMP Assay forJournal of fungi (Basel, Switzerland) · 2024Article
- A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic.Heliyon · 2024Article
- The Role and Value of Professional Rapid Testing of Acute Respiratory Infections (ARIs) in Europe: A Special Focus on the Czech Republic, Poland, and Romania.Diagnostics (Basel, Switzerland) · 2024Review
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- COVID-19 and Water Variables: Review and Scientometric Analysis.International journal of environmental research and public health · 2023Review
- Advances in virus detection methods for wastewater-based epidemiological applications.Case studies in chemical and environmental engineering · 2022Article
- Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care Platform.Food and environmental virology · 2022Article
- Article
- Elucidating the role of environmental management of forests, air quality, solid waste and wastewater on the dissemination of SARS-CoV-2.Hygiene and environmental health advances · 2022Review
- Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality.International journal of environmental research and public health · 2022Review
- Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2.Micromachines · 2022Review
- CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety.Trends in food science & technology · 2022Review
- Label-free sensing of virus-like particles below the sub-diffraction limit by wide-field photon state parametric imaging of a gold nanodot array.Nanoscale advances · 2021Article
- On the Critical Role of Human Feces and Public Toilets in the Transmission of COVID-19: Evidence from China.Sustainable cities and society · 2021Article
- A Portable Device for LAMP Based Detection of SARS-CoV-2.Micromachines · 2021Article
- Loop-Mediated Isothermal Amplification in a Core-Shell Bead Assay for the Detection of Tyrosine Kinase AXL Overexpression.Micromachines · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel corona virus SARS-CoV-2, causing coronavirus disease 2019 (COVID-19), has become a global health challenge particularly for developing countries like Pakistan where overcrowded cities, inadequate sanitation, little health awareness and poor socioeconomic conditions exist. The SARS-CoV-2 has been known to spread primarily through direct contact and respiratory droplets. However, detection of SARS-CoV-2 in stool and sewage have raised the possibility of fecal-oral mode of transmission. Currently, quantitative reverse-transcriptase PCR (qRT-PCR) is the only method being used for SARS-CoV-2 detection, which requires expensive instrumentation, dedicated laboratory setup, highly skilled staff, and several hours to report results. Considering the high transmissibility and rapid spread, a robust, sensitive, specific and cheaper assay for rapid SARS-CoV-2 detection is highly needed. Herein, we report a novel colorimetric RT-LAMP assay for naked-eye detection of SARS-COV-2 in clinical as well as sewage samples. Our SARS-CoV-2 RdRp-based LAMP assay could successfully detect the virus RNA in 26/28 (93%) of RT-PCR positive COVID-19 clinical samples with 100% specificity (n = 7) within 20 min. We also tested the effect of various additives on the performance of LAMP assay and found that addition of 1 mg/ml bovine serum albumin (BSA) could increase the sensitivity of assay up to 10
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