Evidence map›Paper›PMID 34146095›Full record

ArticleThe Journal of heredity2021

Draft de novo Genome Assembly of the Elusive Jaguarundi, Puma yagouaroundi.

Gaik Tamazian, Pavel Dobrynin, Anna Zhuk, Daria V Zhernakova, Polina L Perelman, Natalia A Serdyukova, Alexander S Graphodatsky, Aleksey Komissarov, Sergei Kliver, Nikolay Cherkasov and 5 more

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of heredity, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Genetic rescue of Florida panthers reduced homozygosity but did not swamp ancestral genotypes.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 4 countries.

Gaik TamazianFaculty of Biology, Saint Petersburg State University, St. Petersburg, Russia.ORCID 0000-0002-2931-1123
Pavel DobryninComputer Technologies Laboratory, ITMO University, St. Petersburg, Russia.
Anna ZhukComputer Technologies Laboratory, ITMO University, St. Petersburg, Russia.
Daria V ZhernakovaComputer Technologies Laboratory, ITMO University, St. Petersburg, Russia.
Polina L PerelmanInstitute of Molecular and Cellular Biology, Novosibirsk, Russia.
Natalia A SerdyukovaInstitute of Molecular and Cellular Biology, Novosibirsk, Russia.
Alexander S GraphodatskyInstitute of Molecular and Cellular Biology, Novosibirsk, Russia.
Aleksey KomissarovApplied Genomics Laboratory, SCAMT Institute, ITMO University, St. Petersburg, Russia.
Sergei KliverInstitute of Molecular and Cellular Biology, Novosibirsk, Russia.
Nikolay CherkasovFaculty of Biology, Saint Petersburg State University, St. Petersburg, Russia.
Alan F ScottGenetic Resources Core Facility, McKusick-Nathans Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
David W MohrGenetic Resources Core Facility, McKusick-Nathans Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Klaus-Peter KoepfliSmithsonian-Mason School of Conservation, Front Royal, VA, USA.
Stephen J O'BrienComputer Technologies Laboratory, ITMO University, St. Petersburg, Russia.
Ksenia KrasheninnikovaComputer Technologies Laboratory, ITMO University, St. Petersburg, Russia.ORCID 0000-0002-0604-2047
ITMO University · RUInstitute of Molecular and Cell Biology · RUJohns Hopkins University · USSt Petersburg University · RUSmithsonian Conservation Biology Institute · USUniversity Medical Center Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Puma lineage within the family Felidae consists of 3 species that last shared a common ancestor around 4.9 million years ago. Whole-genome sequences of 2 species from the lineage were previously reported: the cheetah (Acinonyx jubatus) and the mountain lion (Puma concolor). The present report describes a whole-genome assembly of the remaining species, the jaguarundi (Puma yagouaroundi). We sequenced the genome of a male jaguarundi with 10X Genomics linked reads and assembled the whole-genome sequence. The assembled genome contains a series of scaffolds that reach the length of chromosome arms and is similar in scaffold contiguity to the genome assemblies of cheetah and puma, with a contig N50 = 100.2 kbp and a scaffold N50 = 49.27 Mbp. We assessed the assembled sequence of the jaguarundi genome using BUSCO, aligned reads of the sequenced individual and another published female jaguarundi to the assembled genome, annotated protein-coding genes, repeats, genomic variants and their effects with respect to the protein-coding genes, and analyzed differences of the 2 jaguarundis from the reference mitochondrial genome. The jaguarundi genome assembly and its annotation were compared in quality, variants, and features to the previously reported genome assemblies of puma and cheetah. Computational analyzes used in the study were implemented in transparent and reproducible way to allow their further reuse and modification.

Indexed as

FelidaePumaAnimalsFemaleGenomeGenomicsMaleMolecular Sequence Annotation10X Genomics ChromiumFelidaegenome annotationlinked readsPuma yagouaroundiwhole genome assembly

Identifiers

PMID34146095
PMCPMC8558579
OpenAlexW3167793888

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.