Evidence map›Paper›PMID 34143322›Full record

ArticleJournal of molecular histology2021

Circ_PRKDC knockdown promotes skin wound healing by enhancing keratinocyte migration via miR-31/FBN1 axis.

Dawei Han, Wenhui Liu, Guangshuai Li, Linbo Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
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  13. [Research advances on the mechanism of circular RNA in diabetic wound healing].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2023
    Article
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  17. Article
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  19. Beyond the Code: Noncoding RNAs in Skin Wound Healing.Cold Spring Harbor perspectives in biology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Dawei HanDepartment of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
Wenhui LiuDepartment of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
Guangshuai LiDepartment of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
Linbo LiuDepartment of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China. liulinb0@163.com.
First Affiliated Hospital of Zhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circular RNA protein kinase, DNA-activated, catalytic subunit (circ_PRKDC) has been found to impede wound healing in diabetic foot ulcers via regulating keratinocyte proliferation and migration. However, the mechanisms underlying circ_PRKDC in skin wound healing remain unclear. The expression of circ_PRKDC, microRNA (miR)-31 and fibrillin 1 (FBN1) was detected using quantitative reverse transcription-polymerase chain reaction and Western blot assays. The migration ability and the changes of matrix metallopeptidase 9 (MMP-9) and MMP2 levels were determined using wound healing, transwell and Western blot assays. The interaction between miR-31 and circ_PRKDC or FBN1 was verified by dual-luciferase reporter assay. The expression of circ_PRKDC was gradually down-regulated in wound edge at 1 and 7 days after injury relative to the unwounded skin. In human epidermal keratinocytes (HEKa), knockdown of circ_PRKDC promoted cell migration partly through up-regulating MMP-2 and MMP9, while circ_PRKDC overexpression showed opposite effects. In a mechanical study, we confirmed that miR-31 was a target of circ_PRKDC, and inhibition of miR-31 reversed the promotive effect of circ_PRKDC knockdown on HEKa migration. Besides that, miR-31 was verified to target FBN1, and ectopic overexpression of miR-31 accelerated HEKa migration via FBN1. Importantly, we also demonstrated that FBN1 overexpression attenuated the effects of circ_PRKDC knockdown on HEKa migration. In all, circ_PRKDC knockdown promoted HEKa migration during wound healing through miR-31/FBN1 axis, suggesting the therapeutic potential for circ_PRKDC on skin wound healing.

Indexed as

AdipokinesBlotting, WesternCell MovementDNA-Activated Protein KinaseFibrillin-1Gene Knockdown TechniquesHumansKeratinocytesMatrix Metalloproteinase 2Matrix Metalloproteinase 9MicroRNAsReal-Time Polymerase Chain ReactionRNA, CircularRNA, MessengerSkinTransfectionAdipokinesDNA-Activated Protein KinaseFBN1 protein, humanFibrillin-1Matrix Metalloproteinase 2Matrix Metalloproteinase 9MicroRNAsMIRN31 microRNA, humanMMP2 protein, humanMMP9 protein, humanPRKDC protein, humanRNA, CircularRNA, Messengercirc_PRKDCFBN1KeratinocytemiR-31Wound healing

Identifiers

PMID34143322
OpenAlexW3169847984

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.