Evidence map›Paper›PMID 34141498›Full record

ArticleApplications in plant sciences2021

Polishing entire stems and roots using sandpaper under water: An alternative method for macroscopic analyses.

Antonio C F Barbosa, Caian S Gerolamo, André C Lima, Veronica Angyalossy, Marcelo R Pace

Abstract read
In one paragraph

Article in Applications in plant sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Antonio C F BarbosaLaboratório de Madeira e Produtos Derivados Centro de Tecnologia de Recursos Florestais Instituto de Pesquisas Tecnológicas Av. Prof. Almeida Prado 532, Cidade Universitária São Paulo 05508-901 Brazil.
Caian S GerolamoLaboratório de Anatomia Vegetal Instituto de Biociências Departamento de Botânica Universidade de São Paulo Rua do Matão 277, Cidade Universitária São Paulo 05508-090 Brazil.ORCID https://orcid.org/0000-0003-1819-5371
André C LimaWeizmann Tree Lab Department of Plant and Environmental Sciences Weizmann Institute of Science Herzl Street 234 Rehovot 76100 Israel.ORCID https://orcid.org/0000-0001-9183-3064
Veronica AngyalossyLaboratório de Anatomia Vegetal Instituto de Biociências Departamento de Botânica Universidade de São Paulo Rua do Matão 277, Cidade Universitária São Paulo 05508-090 Brazil.ORCID https://orcid.org/0000-0002-9823-9946
Marcelo R PaceDepartamento de Botánica Instituto de Biología Universidad Nacional Autónoma de México Circuito Zona Deportiva s/n de Ciudad Universitaria Mexico City 04510 Mexico.ORCID https://orcid.org/0000-0003-0368-2388

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premisePolishing entire stem and root samples is an effective method for studying their anatomy; however, polishing fresh samples to preserve woods with soft tissues or barks is challenging given that soft tissues shrink when dried. We propose sanding fresh or liquid-preserved samples under water as an alternative, given that it preserves all tissues in an intact and clear state. METHODS AND

resultsBy manually grinding the surface of the samples under water using three ascending grits of waterproof sandpapers, an excellent polished sanded surface is obtained. The wood swarf goes into the water without clogging the cell lumina, rendering the surfaces adequate for cell visualization and description. We show results in palms, liana stems, roots, and wood blocks.

conclusionsUsing this simple, inexpensive, rapid technique, it is possible to polish either fresh, dry, or liquid-preserved woody plant samples, preserving the integrity of both the soft and hard tissues and allowing for detailed observations of the stems and roots.

Indexed as

barkdendrochronologyimage visualizationmethodsmicroanatomywood anatomy

Identifiers

PMID34141498
PMCPMC8202830

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.