ArticleScientific reports2021
Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- A fit-for-purpose validation of a commercial radioimmunoassay for measurement of human peripheral oxytocin.Biochemistry and biophysics reports · 2024Article
- Article
- Inaccuracies in plasma oxytocin extraction and enzyme immunoassay techniques.Comprehensive psychoneuroendocrinology · 2023Article
- Similar behavioral but different endocrine responses to conspecific interactions in hand-raised wolves and dogs.iScience · 2023Article
- What are oxytocin assays measuring? Epitope mapping, metabolites, and comparisons of wildtype & knockout mouse urine.Psychoneuroendocrinology · 2022Article
- Salivary oxytocin in autistic patients and in patients with intellectual disability.Frontiers in psychiatry · 2022Article
- Life experience rather than domestication accounts for dogs' increased oxytocin release during social contact with humans.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study human and animal behaviour with minimal disturbance. However, a thorough validation of analytical methods and an assessment of the physiological significance of these measures are essential. We conducted an analytical validation of a commercial Enzyme Immunoassay (EIA; Arbor OT assay kit) to measure OTM concentrations in dog, wolf, and human urine samples. To test the assay's ability to detect changes in OTM concentrations, we administered oxytocin intranasally to 14 dogs. Assay performance with regard to parallelism was acceptable. Assay accuracy and extraction efficiency for dog and wolf samples were comparable to a previously validated assay (Enzo OT assay kit) but variation was smaller for human samples. Binding sensitivity and antibody specificity were better in the Arbor assay. Average OTM concentrations were more than twice as high as in comparable samples measured with the Enzo assay, highlighting a lack of comparability of absolute values between different assays. Changes in OTM concentrations after intranasal treatment were detected reliably. The Arbor assay met requirements of a "fit-for-purpose" validation with improvement of several parameters compared to the Enzo assay.
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Registered trials
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