ArticleScientific reports2021
Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 37 citations in OpenAlex.
- Partial purification and characterization of an extracellular cold-active protease from Flavobacterium azizsancarii, a novel Antarctic isolate.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Metagenomic analysis of bacterial diversity, antibiotic resistance, and functional profiles in the ice core samples from two glaciers of Sikkim Himalaya.Scientific reports · 2026Article
- Enzymatic valorization of fishery by-products: harnessing cold-adapted marine proteases for sustainable production of bioactive peptides.Archives of microbiology · 2025Review
- Biochemical features and biotechnological potential of a proteolytic extract from a psychrophilic Antarctic bacterium.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Cold-active microbial enzymes and their biotechnological applications.Microbial biotechnology · 2024Review
- Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822FEBS open bio · 2023Article
- Optimization of subtilisin production fromSaudi journal of biological sciences · 2023Article
- Cold-Adapted Proteases: An Efficient and Energy-Saving Biocatalyst.International journal of molecular sciences · 2023Review
- Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya.PloS one · 2023Article
- Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101FEBS open bio · 2022Article
- Molecular characterization of lipase from a psychrotrophic bacterium Pseudomonas sp. CRBC14.Current genetics · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4-30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5-35 °C and pH 6.0-12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low K
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