Evidence map›Paper›PMID 34139881›Full record

ArticleStem cells and development2021

Induction of Ventral Spinal V0 Interneurons from Mouse Embryonic Stem Cells.

Jennifer Pardieck, Manwal Harb, Shelly Sakiyama-Elbert

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jennifer PardieckDepartment of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA.ORCID 0000-0003-2987-6885
Manwal HarbDepartment of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA.
Shelly Sakiyama-ElbertDepartment of Biomedical Engineering, University of Texas at Austin, Austin, Texas, USA.ORCID 0000-0003-4832-5851
The University of Texas at Austin · USWashington University in St. Louis · US

Funding

DEVELOPING NEW TOOLS TO UNDERSTAND THE ROLE OF INTERNEURONS IN REWIRING AFTER SPINAL CORD INJURY.R01NS090617 · NINDS · WASHINGTON UNIVERSITY · PI SAKIYAMA-ELBERT, SHELLY ELESE · 2015 to 2019
$1.6M
Determining the Potential of V0 Interneurons in Promoting Functional Recovery After Spinal Cord Injury in a Rat ModelF31NS100432 · NINDS · UNIVERSITY OF TEXAS AT AUSTIN · PI PARDIECK, JENNIFER · 2017 to 2017
$35k
NINDS NIH HHS F31 NS100432NINDS NIH HHS R01 NS090617
6 · The paper itself

Abstract

The ventral spinal population of V0 interneurons (INs) contributes to the coordinated movements directed by spinal central pattern generators (CPGs), including respiratory circuits and left-right alternation in locomotion. One challenge in studying V0 INs has been the limited number of cells that can be isolated from primary sources for basic research or therapeutic use. However, derivation from a pluripotent source, such as has been done recently for other IN populations, could resolve this issue. However, there is currently no protocol to specifically derive V0 interneurons from pluripotent cell types. To generate an induction protocol, mouse embryonic stem cells (mESCs) were grown in suspension culture and then exposed to retinoic acid (RA) and collected at different time points to measure mRNA expression of the V0 progenitor transcription factor marker,

Indexed as

Mouse Embryonic Stem CellsSpinal CordAnimalsHedgehog ProteinsHomeodomain ProteinsInterneuronsLocomotionMiceDbx1 protein, mouseEvx1 protein, mouseHedgehog ProteinsHomeodomain Proteinscentral pattern generatorinduction protocolmotor controlspinal cord injurytranscription factorventral patterning

Identifiers

PMID34139881
PMCPMC8403187
OpenAlexW3166389342

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.