Evidence map›Paper›PMID 34139267›Full record

ArticleJournal of neuroscience methods2021

Creation and validation of 3D-printed head molds for stereotaxic injections of neonatal mouse brains.

Ethan Chervonski, Asa A Brockman, Rohit Khurana, Yuhao Chen, Sabrina Greenberg, Madeleine S Hay, Yifu Luo, Jason Miller, Deanna Patelis, Sarah K Whitney and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Ethan ChervonskiDepartment of Cell and Developmental Biology, Vanderbilt University, USA.
Asa A BrockmanDepartment of Cell and Developmental Biology, Vanderbilt University, USA.
Rohit KhuranaDepartment of Cell and Developmental Biology, Vanderbilt University, USA.
Yuhao ChenDepartment of Biomedical Engineering, Vanderbilt University, USA.
Sabrina GreenbergDepartment of Biomedical Engineering, Vanderbilt University, USA.
Madeleine S HayDepartment of Biomedical Engineering, Vanderbilt University, USA.
Yifu LuoDepartment of Biomedical Engineering, Vanderbilt University, USA.
Jason MillerDepartment of Biomedical Engineering, Vanderbilt University, USA.
Deanna PatelisDepartment of Biomedical Engineering, Vanderbilt University, USA.
Sarah K WhitneyDepartment of Biomedical Engineering, Vanderbilt University, USA.
Matthew WalkerDepartment of Biomedical Engineering, Vanderbilt University, USA.
Rebecca A IhrieDepartment of Cell and Developmental Biology, Vanderbilt University, USA; Department of Neurological Surgery, Vanderbilt University Medical Center, USA. Electronic address: rebecca.ihrie@vanderbilt.edu.
Vanderbilt University · US

Funding

Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MATTHEW J TYSKA · 1995 to 2026
$172.8M
Translational Analysis CoreP30DK058404 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI RICHARD M. PEEK · 2002 to 2026
$29.9M
Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI OWEN P MCGUINNESS · 2012 to 2026
$29.3M
Shop Module CoreP30EY008126 · NEI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Kevin L Schey · 1989 to 2026
$19.6M
Vanderbilt Mouse Metabolic Physiology CenterU24DK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2001 to 2015
$14.9M
Overall: Eunice Kennedy Shriver Intellectual and Developmental Disabilities Research Center at VanderbiltP50HD103537 · NICHD · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Lea K Davis · 2020 to 2026
$10.3M
Quantifying Differences in mTOR Activity and Tumor Development Between Neural Stem Cell MicrodomainsR01NS096238 · NINDS · VANDERBILT UNIVERSITY · PI IHRIE, REBECCA A · 2016 to 2020
$1.7M
LSM880-Airyscan Confocal Microscope for Large Shared ResourceS10OD021630 · OD · VANDERBILT UNIVERSITY · PI WELLS, K. SAM · 2016 to 2016
$560k
NCI NIH HHS P30 CA068485NEI NIH HHS P30 EY008126NICHD NIH HHS P50 HD103537NIDDK NIH HHS P30 DK020593NIDDK NIH HHS P30 DK058404NIDDK NIH HHS U24 DK059637NIH HHS S10 OD021630NINDS NIH HHS R01 NS096238
6 · The paper itself

Abstract

backgroundAn increasing number of rodent model systems use injection of DNA or viral constructs in the neonatal brain. However, approaches for reliable positioning and stereotaxic injection at this developmental stage are limited, typically relying on handheld positioning or molds that must be re-aligned for use in a given laboratory. NEW

methodA complete protocol and open-source software pipeline for generating 3D-printed head molds derived from a CT scan of a neonatal mouse head cast, together with a universal adapter that can be placed on a standard stereotaxic stage.

resultsA series of test injections with adenovirus encoding red fluorescent protein, or Fluorogold, were conducted using original clay molds and newly generated 3D printed molds. Several metrics were used to compare spread and localization of targeted injections. COMPARISON WITH EXISTING

methodsThe new method of head mold generation gave comparable results to the field standard, but also allowed the rapid generation of additional copies of each head mold with standardized positioning of the head each time.

conclusionsThis 3D printing pipeline can be used to efficiently develop a series of head molds with standardized injection coordinates across multiple laboratories. More broadly, this pipeline can easily be adapted to other perinatal ages or species.

Indexed as

Imaging, Three-DimensionalPrinting, Three-DimensionalAnimalsAnimals, NewbornBrainMiceTomography, X-Ray Computed3D printingLineage tracingNeonatal brainNeural stem cellsRadial gliaStereotaxic injectionVentricular-subventricular zoneViral injection

Identifiers

PMID34139267
PMCPMC8324552
OpenAlexW3170084602

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.