ArticleJournal of medical virology2021
Infectivity assay for detection of SARS-CoV-2 in samples from patients with COVID-19.
Article in Journal of medical virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Oral mouthwashes for asymptomatic to mildly symptomatic adults with COVID-19 and salivary viral load: a randomized, placebo-controlled, open-label clinical trial.BMC oral health · 2024Trial
- Trial
- Diagnostic Value and Outcomes of Systematic SARS-CoV-2 Screening in Asymptomatic Patients.JAMA network open · 2026Article
- Impact of Vaccination and Prior Infection on SARS-CoV-2 Viral Load in Preschool Children During the Omicron Pandemic.Vaccines · 2025Article
- Investigating the effectiveness of commercially available mouthwash on SARS-CoV-2 in vivo using viable virus titre as the primary outcome. A randomised controlled trial.Access microbiology · 2024Article
- Comparison of SARS-CoV-2 molecular results from the first two COVID-19 waves in Gauteng.Southern African journal of infectious diseases · 2024Article
- Efficient Direct and Limited Environmental Transmission of SARS-CoV-2 Lineage B.1.22 in Domestic Cats.Microbiology spectrum · 2023Article
- COVID-19 relapse associated with SARS-CoV-2 evasion from CD4iScience · 2023Article
- SARS-CoV-2 antigen tests for screening of healthcare workers; experience with over 48,000 combined antigen tests and RT-PCR tests.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2023Article
- Determining the impact of vaccination on SARS-CoV-2 RT-PCR cycle threshold values and infectious viral titres.Access microbiology · 2023Article
- A comparison of SARS-CoV-2 RNA extraction with the QuickGene-810 Nucleic Acid Isolation System compared to the EZ1 Advanced DSP Virus Kit.Access microbiology · 2022Article
- Factors associated with weak positive SARS-CoV-2 diagnosis by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR).Pathology · 2022Article
- COVID-19 active case findings based on self-collected saliva samples with CRISPR-Cas12a detection.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- Evaluating the Ability to ID (COVID-19) NOW: a Large Real-World Prospective Evaluation of the Abbott ID NOW COVID-19 Assay.Microbiology spectrum · 2022Article
- Anti-nucleocapsid antibodies enhance the production of IL-6 induced by SARS-CoV-2 N protein.Scientific reports · 2022Article
- Stability of SARS-CoV-2 and influenza virus varies across different paper types.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2022Article
- Severe Acute Respiratory Syndrome Coronavirus 2 Is Detected in the Gastrointestinal Tract of Asymptomatic Endoscopy Patients but Is Unlikely to Pose a Significant Risk to Healthcare Personnel.Gastro hep advances · 2022Article
- Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review.Bioengineered · 2021Review
- Infectivity assay for detection of SARS-CoV-2 in samples from patients with COVID-19.Journal of medical virology · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Since the coronavirus disease 2019 (COVID-19) outbreak, laboratory diagnosis has mainly been conducted using reverse-transcription polymerase chain reaction (RT-PCR). Detecting the presence of an infectious virus in the collected sample is essential to analyze if a person can transmit infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, there have been no quantitative investigations conducted for infectious SARS-CoV-2 in clinical samples. Therefore, in the present study, a rapid and simple focus-forming assay using the peroxidase-antiperoxidase technique was developed to quantify infectious SARS-CoV-2 titers in 119 samples (n = 52, nasopharyngeal swabs [NPS]; n = 67, saliva) from patients with COVID-19. Furthermore, the study findings were compared with the cycle threshold (Ct) values of real-time RT-PCR. The infectious virus titers in NPS samples and Ct values were inversely correlated, and no infectious virus could be detected when the Ct value exceeded 30. In contrast, a low correlation was observed between the infectious virus titers in saliva and Ct values (r = -0.261, p = 0.027). Furthermore, the infectious virus titers in the saliva were significantly lower than those in the NPS samples. Ten days after the onset of COVID-19 symptoms, the infectious virus was undetectable, and Ct values were more than 30 in NSP and saliva samples. The results indicate that patients whose symptoms subsided 10 days after onset, with Ct values more than 30 in NSP and saliva samples, were less likely to infect others.
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