ArticleFood science & nutrition2021
Improved triplex real-time PCR with endogenous control for synchronous identification of DNA from chicken, duck, and goose meat.
Article in Food science & nutrition, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Poultry Universal Primer-Based Fluorescent PCR (PUP-fPCR) for Simultaneous Identification and Quantification of Chicken, Quail, Duck, and Goose Meat Species.Molecules (Basel, Switzerland) · 2026Article
- A Multiplex Real-Time PCR for Qualitative Detection of Mutton, Pork, Chicken, and Duck in Processed Meat Products.Food science & nutrition · 2025Article
- Mammalian and avian species quantification in homogenized foods: real time PCR and digital PCR as tools for label compliance controls.Scientific reports · 2024Article
- Rapid detection of duck ingredient in adulterated foods by isothermal recombinase polymerase amplification assays.Food chemistry. Molecular sciences · 2023Article
- A Novel Universal Primer Multiplex Real-Time PCR (UP-M-rtPCR) Approach for Specific Identification and Quantitation of Cat, Dog, Fox, and Mink Fractions Using Nuclear DNA Sequences.Foods (Basel, Switzerland) · 2023Article
- Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD.Current research in food science · 2023Article
- A Heptaplex PCR Assay for Molecular Traceability of Species Origin With High Efficiency and Practicality in Both Raw and Heat Processing Meat Materials.Frontiers in nutrition · 2022Article
- Molecular Authentication of Twelve Meat Species Through a Promising Two-Tube Hexaplex Polymerase Chain Reaction Technique.Frontiers in nutrition · 2022Article
- Pre-Degassed Microfluidic Chamber-Based Digital PCR Device for Meat Authentication Applications.Micromachines · 2021Article
- Improved triplex real-time PCR with endogenous control for synchronous identification of DNA from chicken, duck, and goose meat.Food science & nutrition · 2021Article
- A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species.Foods (Basel, Switzerland) · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The authentication and labeling of meat products, concerning origins and species, are key to fair trade and to protect consumer interests in the market. We developed an improved triplex real-time PCR approach to simultaneously identify chicken, duck, and goose DNA in meat, including an endogenous control to avoid false negatives. Our method specifically detected DNA from chicken, duck, and goose, and showed no cross-reaction with DNA extracted from other meat types. The detection limits of chicken, duck, and goose DNA were 0.001-0.00025 ng, 0.0025-0.0001 ng, and 0.001-0.00001 ng, respectively, and we were able to simultaneously identify DNA from two distinct origins using as little as 0.1% of total meat weight. Our newly generated triplex real-time PCR method with endogenous control exhibited high specificity, sensitivity, and efficiency for simultaneous identification of DNA from chicken, duck, and goose in meat.
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