ArticleScientific reports2021
Enhanced lipid production by addition of malic acid in fermentation of recombinant Mucor circinelloides Mc-MT-2.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 23 citations in OpenAlex.
- Recent advances and future directions on GLA-producing organisms.Frontiers in bioengineering and biotechnology · 2025Review
- Potential of oleaginous microbes for lipid accumulation and renewable energy generation.World journal of microbiology & biotechnology · 2024Review
- Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies.Archives of microbiology · 2024Review
- Simultaneous production of high-value lipids in Schizochytrium sp. by synergism of chemical modulators.Applied microbiology and biotechnology · 2023Article
- Controlling of Mycobacterium by Natural Degradant-Combination Models for Sequestering Mycolic Acids in Karish Cheese.Molecules (Basel, Switzerland) · 2022Article
- Study on the chemical compositions and microbial communities of cigar tobacco leaves fermented with exogenous additive.Scientific reports · 2022Article
- Investigating the Effect of Alcohol Dehydrogenase Gene Knockout on Lipid Accumulation inJournal of fungi (Basel, Switzerland) · 2022Article
- Homologous and Heterologous Expression of Delta(12)-Desaturase inMolecules (Basel, Switzerland) · 2022Article
- Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid inFrontiers in microbiology · 2022Article
- Transcriptome analysis of malate-inducedFrontiers in microbiology · 2022Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In our previous work, we reported a novel approach for increasing lipid production in an oleaginous fungus Mucor circinelloides by overexpression of mitochondrial malate transporter protein. This transporter plays a vital role in fatty acid biosynthesis during malate and citrate transport systems in oleaginous fungi. In this study, the controlling metabolic supplementation strategy was used to improve the lipid production by overexpression of malate transporter protein in M. circinelloides strain coded as Mc-MT-2. The effects of different metabolic intermediates on lipid production in batch fermentation by Mc-MT-2 were investigated. The optimal lipid production was obtained at 0.8% malic acid after 24 h of fermentation. Furthermore, in fed-batch bioreactors containing glucose as a carbon source supplemented with malic acid, the highest cell growth, and lipid production were achieved. The resulting strain showed the fungal dry biomass of 16 g/L, a lipid content of 32%, lipid yield of 5.12 g/L in a controlled bench-top bioreactor, with 1.60-, 1.60- and 2.56-fold improvement, respectively, compared with the batch control without supplementation of malic acid. Our findings revealed that the addition of malic acid during fermentation might play an important role in lipid accumulation in the recombinant M. circinelloides Mc-MT-2. This study provides valuable insights for enhanced microbial lipid production through metabolic supplementation strategy in large scale and industrial applications.
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