Evidence map›Paper›PMID 34135099›Full record

ArticleLife science alliance2021

Dissection-independent production of

Joshua Blight, Katarzyna A Sala, Erwan Atcheson, Holger Kramer, Aadil El-Turabi, Eliana Real, Farah A Dahalan, Paulo Bettencourt, Emma Dickinson-Craig, Eduardo Alves and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life science alliance, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. An OptimizedBio-protocol · 2024
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 3 countries.

Joshua BlightDepartment of Life Sciences, Imperial College London, Sir Alexander Fleming Building, London, UK.ORCID 0000-0003-3624-7008
Katarzyna A SalaDepartment of Life Sciences, Imperial College London, Sir Alexander Fleming Building, London, UK.
Erwan AtchesonThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.
Holger KramerDepartment of Physiology, Anatomy and Genetics, Henry Wellcome Building for Gene Function, University of Oxford, Oxford, UK.ORCID 0000-0002-6400-0945
Aadil El-TurabiThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.ORCID 0000-0003-1092-8155
Eliana RealDepartment of Life Sciences, Imperial College London, Sir Alexander Fleming Building, London, UK.
Farah A DahalanDepartment of Life Sciences, Imperial College London, Sir Alexander Fleming Building, London, UK.
Paulo BettencourtThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.ORCID 0000-0002-4884-5470
Emma Dickinson-CraigThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.
Eduardo AlvesThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.
Ahmed M SalmanThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.
Chris J JanseDepartment of Parasitology, Leiden Malaria Research Group, Center of Infectious Diseases, Leiden University Medical Center, (LUMC, L4-Q), Leiden, The Netherlands.ORCID 0000-0002-6410-6205
Frances M AshcroftDepartment of Physiology, Anatomy and Genetics, Henry Wellcome Building for Gene Function, University of Oxford, Oxford, UK.ORCID 0000-0002-6970-1767
Adrian Vs HillThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.ORCID 0000-0003-0900-9629
Arturo Reyes-SandovalThe Jenner Institute, University of Oxford, Old Road Campus Research Building, Oxford, UK.ORCID 0000-0002-2648-1696
Andrew M BlagboroughDepartment of Life Sciences, Imperial College London, Sir Alexander Fleming Building, London, UK.
Jake BaumDepartment of Life Sciences, Imperial College London, Sir Alexander Fleming Building, London, UK jake.baum@imperial.ac.uk.ORCID 0000-0002-0275-352X
University of Oxford · GBImperial College London · GBCentre for Human Genetics · GBLeiden University Medical Center · NLUniversity of Cambridge · GB

Funding

Medical Research Council 1240480Medical Research Council MC_PC_18050Medical Research Council MR/N00227X/1Wellcome TrustWellcome Trust 04931/Z/14/ZWellcome Trust 084655/Z/08/ZWellcome Trust 097395/Z/11/ZWellcome Trust 100993/Z/13/Z
6 · The paper itself

Abstract

Progress towards a protective vaccine against malaria remains slow. To date, only limited protection has been routinely achieved following immunisation with either whole-parasite (sporozoite) or subunit-based vaccines. One major roadblock to vaccine progress, and to pre-erythrocytic parasite biology in general, is the continued reliance on manual salivary gland dissection for sporozoite isolation from infected mosquitoes. Here, we report development of a multi-step method, based on batch processing of homogenised whole mosquitoes, slurry, and density-gradient filtration, which combined with free-flow electrophoresis rapidly produces a pure, infective sporozoite inoculum. Human-infective

Indexed as

AnimalsCulicidaeDisease Models, AnimalDrosophilaHep G2 CellsHumansImmunizationMalariaMalePlasmodium bergheiPlasmodium falciparumRatsSporozoites

Identifiers

PMID34135099
PMCPMC8321652
OpenAlexW3171943231

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.