Evidence map›Paper›PMID 34134982›Full record

ArticleScience advances2021

Longitudinal high-resolution imaging through a flexible intravital imaging window.

Guillaume Jacquemin, Maria Benavente-Diaz, Samir Djaber, Aurélien Bore, Virginie Dangles-Marie, Didier Surdez, Shahragim Tajbakhsh, Silvia Fre, Bethan Lloyd-Lewis

Abstract read
In one paragraph

Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023
    Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Monitoring and modulation of the tumor microenvironment for enhanced cancer modeling.Experimental biology and medicine (Maywood, N.J.) · 2022
    Review
  15. Article
  16. Longitudinal Intravital Imaging Through Clear Silicone Windows.Journal of visualized experiments : JoVE · 2022
    Article
  17. Article
  18. Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Guillaume JacqueminInstitut Curie, Laboratory of Genetics and Developmental Biology, PSL Research University, INSERM U934, CNRS UMR3215, F-75248 Paris Cedex 05, France. guillaume.jacquemin@curie.fr silvia.fre@curie.fr bethan.lloyd-lewis@bristol.ac.uk.ORCID http://orcid.org/0000-0001-7542-5490
Maria Benavente-DiazStem Cells & Development Unit, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, France.ORCID http://orcid.org/0000-0002-6419-4698
Samir DjaberInstitut Curie, Laboratory of Genetics and Developmental Biology, PSL Research University, INSERM U934, CNRS UMR3215, F-75248 Paris Cedex 05, France.
Aurélien BoreInstitut Curie, Laboratory of Genetics and Developmental Biology, PSL Research University, INSERM U934, CNRS UMR3215, F-75248 Paris Cedex 05, France.ORCID http://orcid.org/0000-0003-1665-5629
Virginie Dangles-MarieFaculty of Pharmacy, Université Paris Descartes, Paris, France.ORCID http://orcid.org/0000-0003-0845-2936
Didier SurdezINSERM U830, Équipe Labellisée LNCC, Diversity and Plasticity of Childhood Tumors Lab, PSL Research University, SIREDO Oncology Centre, Institut Curie Research Centre, Paris, France.ORCID http://orcid.org/0000-0002-7118-7859
Shahragim TajbakhshStem Cells & Development Unit, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, France.ORCID http://orcid.org/0000-0002-5978-2057
Silvia FreInstitut Curie, Laboratory of Genetics and Developmental Biology, PSL Research University, INSERM U934, CNRS UMR3215, F-75248 Paris Cedex 05, France. guillaume.jacquemin@curie.fr silvia.fre@curie.fr bethan.lloyd-lewis@bristol.ac.uk.ORCID http://orcid.org/0000-0002-7209-7636
Bethan Lloyd-LewisInstitut Curie, Laboratory of Genetics and Developmental Biology, PSL Research University, INSERM U934, CNRS UMR3215, F-75248 Paris Cedex 05, France. guillaume.jacquemin@curie.fr silvia.fre@curie.fr bethan.lloyd-lewis@bristol.ac.uk.ORCID http://orcid.org/0000-0001-6511-1818

Funding

Wellcome Trust
6 · The paper itself

Abstract

Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, sutureless design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening previously unattainable avenues for in vivo imaging of soft and fragile tissues.

Identifiers

PMID34134982
PMCPMC8208712

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.