ArticleCell2021
Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
Article in Cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 119 citations in OpenAlex.
- α-Parvin regulation of cell rearrangement is critical for ureteric bud branching morphogenesis.Science advances · 2026Article
- Prepatterned Tissue Stiffness Gradient Controls Organ shape and size.bioRxiv : the preprint server for biology · 2026Article
- The instructive scar - the dynamic role of extracellular matrix in biliary pathophysiology.Disease models & mechanisms · 2026Review
- Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface.Npj imaging · 2026Article
- Mechanisms of active wetting and fluidification in epithelial cell collectives.Nature materials · 2026Article
- Developmentally inspired synthetic kidney engineering.Nature biotechnology · 2026Review
- Fostering cell-cell interactions and integrating angiocrine factors to promote the development of salivary microtissues in 3D.Bioengineering & translational medicine · 2026Article
- Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.bioRxiv : the preprint server for biology · 2026Article
- Targeted regeneration of post-radiation epithelium without promoting cancer recurrence.Research square · 2026Article
- Notch signaling is a driver of glandular stem cell activity and regenerative migration after damage.The EMBO journal · 2026Article
- Microtubule dependent changes in cell polarity are required for mammary epithelial cell migration and branching morphogenesis.Biology open · 2025Article
- Pan-cancer and machine learning reveal the role of cell adhesion molecules in prognosis, immune remodeling, and anti-tumour therapy in lower-grade gliomas.Discover oncology · 2025Article
- Human iPSC-derived salivary gland organoids model diabetic salivary gland dysfunction.bioRxiv : the preprint server for biology · 2025Article
- Stress relaxing granular bioprinting materials enable complex and uniform organoid self-organization.bioRxiv : the preprint server for biology · 2025Article
- Engineering kidney developmental trajectory using culture boundary conditions.Nature communications · 2025Article
- Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker.ACS nano · 2025Article
- Live imaging of the extracellular matrix with a glycan-binding fluorophore.Nature methods · 2025Article
- Focal adhesion in the tumour metastasis: from molecular mechanisms to therapeutic targets.Biomarker research · 2025Review
- The Mechanics of Building Functional Organs.Cold Spring Harbor perspectives in biology · 2025Review
- Crafting the future of bone regeneration: the promise of supramolecular peptide nanofiber hydrogels.Frontiers in bioengineering and biotechnology · 2025Review
14 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Many embryonic organs undergo epithelial morphogenesis to form tree-like hierarchical structures. However, it remains unclear what drives the budding and branching of stratified epithelia, such as in the embryonic salivary gland and pancreas. Here, we performed live-organ imaging of mouse embryonic salivary glands at single-cell resolution to reveal that budding morphogenesis is driven by expansion and folding of a distinct epithelial surface cell sheet characterized by strong cell-matrix adhesions and weak cell-cell adhesions. Profiling of single-cell transcriptomes of this epithelium revealed spatial patterns of transcription underlying these cell adhesion differences. We then synthetically reconstituted budding morphogenesis by experimentally suppressing E-cadherin expression and inducing basement membrane formation in 3D spheroid cultures of engineered cells, which required β1-integrin-mediated cell-matrix adhesion for successful budding. Thus, stratified epithelial budding, the key first step of branching morphogenesis, is driven by an overall combination of strong cell-matrix adhesion and weak cell-cell adhesion by peripheral epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.