ArticleNucleic acids research2021
RNase H1C collaborates with ssDNA binding proteins WHY1/3 and recombinase RecA1 to fulfill the DNA damage repair in Arabidopsis chloroplasts.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Landscape of plastid DNA breaks in Arabidopsis during development and environmental stimulus.The Plant cell · 2026Article
- Loss of lactate dehydrogenase A disrupts R-loop homeostasis and induces DNA damage in breast cancer cells.Frontiers in genetics · 2026Article
- WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants.Annals of botany · 2024Review
- Genome-Wide Identification and Molecular Evolutionary History of the Whirly Family Genes inPlants (Basel, Switzerland) · 2024Article
- Article
- Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence.Nature communications · 2024Article
- Mechanistic insights into DNA damage recognition and checkpoint control in plants.Nature plants · 2024Review
- Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage.Nature communications · 2024Article
- DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis.Nature communications · 2023Article
- Ribonucleotide and R-Loop Damage in Plastid DNA and Mitochondrial DNA during Maize Development.Plants (Basel, Switzerland) · 2023Article
- WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition.The Biochemical journal · 2023Article
- The Evolutionary Constraints on Angiosperm Chloroplast Adaptation.Genome biology and evolution · 2023Article
- Beyond transcription: compelling open questions in plant RNA biology.The Plant cell · 2023Article
- UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [CaiScience · 2023Article
- WHIRLY protein functions in plants.Food and energy security · 2023Review
- Regulation of Heat Stress inInternational journal of molecular sciences · 2022Article
- WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles.The Biochemical journal · 2022Article
- WHIRLIES Are Multifunctional DNA-Binding Proteins With Impact on Plant Development and Stress Resistance.Frontiers in plant science · 2022Review
- Intragenic tRNA-promoted R-loops orchestrate transcription interference for plant oxidative stress responses.The Plant cell · 2021Article
- Mitochondrial RNase H1 activity regulates R-loop homeostasis to maintain genome integrity and enable early embryogenesis in Arabidopsis.PLoS biology · 2021Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proper repair of damaged DNA is crucial for genetic integrity and organismal survival. As semi-autonomous organelles, plastids have their own genomes whose integrity must be preserved. Several factors have been shown to participate in plastid DNA damage repair; however, the underlying mechanism remains unclear. Here, we elucidate a mechanism of homologous recombination (HR) repair in chloroplasts that involves R-loops. We find that the recombinase RecA1 forms filaments in chloroplasts during HR repair, but aggregates as puncta when RNA:DNA hybrids accumulate. ssDNA-binding proteins WHY1/3 and chloroplast RNase H1 AtRNH1C are recruited to the same genomic sites to promote HR repair. Depletion of AtRNH1C or WHY1/3 significantly suppresses the binding of RNA polymerase to the damaged DNA, thus reducing HR repair and modulating microhomology-mediated double-strand break repair. Furthermore, we show that DNA polymerase IB works with AtRNH1C genetically to complete the DNA damage repair process. This study reveals the positive role of R-loops in facilitating the activities of WHY1/3 and RecA1, which in turn secures HR repair and organellar development.
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