Evidence map›Paper›PMID 34132944›Full record

ArticleMolecular biology reports2021

Evaluation of circulating microRNAs-211 and 25 as diagnostic biomarkers of colorectal cancer.

Eman Radwan, Asmaa S Shaltout, Shima Gafar Mansor, Engy A Shafik, Wael A Abbas, Mahmoud Refaat Shehata, Maha Ali

Abstract read
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In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 2 pooled it
1.0field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 2 syntheses or guidelines pooled it, 15 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
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  5. Review
  6. Article
  7. Review
  8. Review
  9. Dynamic liquid biopsy components as predictive and prognostic biomarkers in colorectal cancer.Journal of experimental & clinical cancer research : CR · 2022
    Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Eman RadwanFaculty of Medicine, Department of Medical Biochemistry, Assiut University, Assiut, 71515, Egypt.
Asmaa S ShaltoutFaculty of Medicine, Department of Microbiology, Assiut University, Assiut, Egypt.
Shima Gafar MansorDepartment of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Engy A ShafikDepartment of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Wael A AbbasFaculty of Medicine, Department of Internal Medicine, Assiut University, Assiut, Egypt.
Mahmoud Refaat ShehataFaculty of Medicine, Department of General Surgery, Assiut University, Assiut, Egypt.
Maha AliFaculty of Medicine, Department of Medical Biochemistry, Assiut University, Assiut, 71515, Egypt. mahaali@aun.edu.eg.ORCID http://orcid.org/0000-0002-3530-4536
Assiut University · EGSphinx University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorectal cancer is one of the most prevalent and deadly cancers worldwide. MicroRNAs are short single stranded non-coding RNAs that play important roles in carcinogenesis, tumor growth and tumor survival. Circulating microRNAs are increasingly becoming efficient and important biomarkers for several types of cancers. Herein, we aim to evaluate the diagnostic potentials of plasma microRNA-211 and microRNA-25 in colorectal cancer patients. Forty-four patients diagnosed with colorectal cancer and 40 healthy controls were recruited for the present study. Expressions of circulating microRNAs -211 and 25 were assessed by quantitative real-time polymerase chain reaction (RT-qPCR). Expression of transforming growth factor-beta, a key factor in tumorigenesis and a key inducer of epithelial to mesenchymal transition was assessed by enzyme-linked immunosorbent assay (ELISA) in patients' tissue and plasma. Our results demonstrated upregulated expressions of plasma microRNAs-211 and 25 correlated with the high transforming growth factor-beta (TGF-β1) expression in patients. In addition, plasma levels were positively correlated with lymph node metastasis. Moreover, receiver operating characteristic analysis demonstrated the reliability of microRNAs-211 and 25 for discriminating colorectal cancer patients from healthy individuals. MicroRNA-211 and microRNA-25 might have a tumorigenic role in colorectal cancer and their plasma levels could be potential biomarkers in its diagnosis.

Indexed as

AgedBiomarkers, TumorColorectal NeoplasmsEnzyme-Linked Immunosorbent AssayEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHumansLymphatic MetastasisMaleMicroRNAsMiddle AgedTransforming Growth Factor beta1Biomarkers, TumorMicroRNAsMIRN211 microRNA, humanMIRN25 microRNA, humanTGFB1 protein, humanTransforming Growth Factor beta1Circulating microRNAsColorectal cancerTGF-β

Identifiers

PMID34132944
OpenAlexW3166312379

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.