Evidence map›Paper›PMID 34132917›Full record

ArticlePlanta2021

A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.).

Rukmini Mishra, Jatindra Nath Mohanty, Bijayalaxmi Mahanty, Raj Kumar Joshi

Abstract read
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In one paragraph

Article in Planta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
10.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 72 citations in OpenAlex.

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  11. Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.).The Plant journal : for cell and molecular biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Rukmini MishraSchool of Applied Science, Centurion University of Technology and Management, Bhubaneswar, 752050, India.
Jatindra Nath MohantyCentre for Biomedical Informatics, Siksha O Anusandhan University, Bhubaneswar, 751003, India.
Bijayalaxmi MahantyDepartment of Biotechnology, Rama Devi Women's University, Vidya Vihar, Bhubaneswar, 751022, India.
Raj Kumar JoshiDepartment of Biotechnology, Rama Devi Women's University, Vidya Vihar, Bhubaneswar, 751022, India. rkjoshi@rdwu.ac.in.ORCID http://orcid.org/0000-0003-0505-2881
Rama Devi Women's University · INCenturion University of Technology and Management · INSiksha O Anusandhan University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MAIN

conclusionT-DNA-free homozygous mutant lines developed through a single transcript CRISPR/Cas9 system harboring the desired modification in the CaERF28 locus exhibited significantly enhanced resistance to the anthracnose pathogen Colletotrichum truncatum coupled with the improved expression of defense responsive genes. Anthracnose, caused by Colletotrichum species, is a major disease of chilli (Capsicum annuum) accounting for significant pre- and post-harvest yield losses across the tropical and subtropical regions of the world. Management of chilli anthracnose using traditional methods have not met with noticeable success. In the present study, we have demonstrated an enhanced anthracnose resistance through a single transcript unit CRISPR/Cas9 mediated alteration of the susceptibility gene CaERF28 in C. annuum. A construct with a single Pol II promoter-driven expression of Cas9, sgRNA and a hammerhead ribozyme (RZ) was designed to modify the CaERF28 gene in the susceptible chilli genotype Arka Lohit. Fourty-five C-ERF28-induced mutant lines (72.5%) were identified from 62 T

Indexed as

CapsicumColletotrichumCRISPR-Cas SystemsMutagenesisPlant DiseasesAnthracnoseCapsicum annuumCRISPR/Cas9Ethylene response factorMutagenesisSingle transcript unit

Identifiers

PMID34132917
OpenAlexW3171319165

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.