ArticleAnalytical and bioanalytical chemistry2021
Nucleic acid lateral flow assay for simultaneous detection of hygiene indicator bacteria.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Polymerase Chain Reaction-Lateral Flow Strip for DetectingAntibiotics (Basel, Switzerland) · 2025Article
- Isothermal Amplification and Hypersensitive Fluorescence Dual-Enhancement Nucleic Acid Lateral Flow Assay for Rapid Detection ofBiosensors · 2023Article
- A universal approach for sensitive and rapid detection of different pathogenic bacteria based on aptasensor-assisted SERS technique.Analytical and bioanalytical chemistry · 2023Article
- Simultaneous and Visual Detection of KPC and NDM Carbapenemase-Encoding Genes Using Asymmetric PCR and Multiplex Lateral Flow Strip.Journal of analytical methods in chemistry · 2023Article
- Equipment-free, salt-mediated immobilization of nucleic acids for nucleic acid lateral flow assays.Sensors and actuators. B, Chemical · 2022Article
- Research progress on detection techniques for point-of-care testing of foodborne pathogens.Frontiers in bioengineering and biotechnology · 2022Review
- Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A simple and rapid polymerase chain reaction (PCR)-based lateral flow assay (LFA) was developed for multiplex detection of hygiene indicator bacteria. Specifically, new PCR primers were designed for accurately detecting Escherichia coli, coliform bacteria, and total bacteria, and the results obtained as a colorimetric signal (generated by the accumulation of gold nanoparticles at distinct test zones on flow strips) could be identified by the naked eye in <10 min after the completion of PCR. The proposed LFA system did not exhibit any cross-reactivities with 8 distinct bacterial strains and can detect down to 1 colony forming unit (CFU)/mL. Furthermore, three species of cultured bacteria (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) inoculated onto sterilized ham were successfully analyzed using the LFA system, which demonstrated that this system shows sufficient sensitivity and specificity for food hygiene monitoring. The speed and simplicity of this LFA make it suitable for use in the food industry as part of routine screening analysis.
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Registered trials
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