Evidence map›Paper›PMID 34131005›Full record

ArticleGenome research2021

Long-read RNA sequencing reveals widespread sex-specific alternative splicing in threespine stickleback fish.

Alice S Naftaly, Shana Pau, Michael A White

Open access · greenAbstract read
In one paragraph

Article in Genome research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 64 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Expression Divergence in Response to Sex-biased Selection.Molecular biology and evolution · 2025
    Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Sex Differences in Cochlear Transcriptomes in Horseshoe Bats.Animals : an open access journal from MDPI · 2024
    Article
  12. Quantifying transcriptome diversity: a review.Briefings in functional genomics · 2024
    Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Alice S NaftalyDepartment of Genetics, University of Georgia, Athens, Georgia 30602, USA.
Shana PauDepartment of Genetics, University of Georgia, Athens, Georgia 30602, USA.
Michael A WhiteDepartment of Genetics, University of Georgia, Athens, Georgia 30602, USA.ORCID 0000-0001-6772-1388
University of Georgia · US

Funding

T32 Predoctoral training grant in geneticsT32GM007103 · NIGMS · UNIVERSITY OF GEORGIA (UGA) · PI DYER, KELLY A · 1985 to 2021
$5.9M
NIGMS NIH HHS T32 GM007103
6 · The paper itself

Abstract

Alternate isoforms are important contributors to phenotypic diversity across eukaryotes. Although short-read RNA-sequencing has increased our understanding of isoform diversity, it is challenging to accurately detect full-length transcripts, preventing the identification of many alternate isoforms. Long-read sequencing technologies have made it possible to sequence full-length alternative transcripts, accurately characterizing alternative splicing events, alternate transcription start and end sites, and differences in UTR regions. Here, we use Pacific Biosciences (PacBio) long-read RNA-sequencing (Iso-Seq) to examine the transcriptomes of five organs in threespine stickleback fish (

Indexed as

Alternative SplicingSmegmamorphaAnimalsGene Expression ProfilingHigh-Throughput Nucleotide SequencingSequence Analysis, RNATranscriptome

Identifiers

PMID34131005
PMCPMC8327910
OpenAlexW3172463310

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.