Evidence map›Paper›PMID 34130086›Full record

ArticleBiosensors & bioelectronics2021

Raman and quantitative phase imaging allow morpho-molecular recognition of malignancy and stages of B-cell acute lymphoblastic leukemia.

Santosh Kumar Paidi, Piyush Raj, Rosalie Bordett, Chi Zhang, Sukrut H Karandikar, Rishikesh Pandey, Ishan Barman

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 40 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. On the use of deep learning for phase recovery.Light, science & applications · 2024
    Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Santosh Kumar PaidiDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Piyush RajDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Rosalie BordettConnecticut Children's Innovation Center, University of Connecticut School of Medicine, Farmington, CT, 06032, USA.
Chi ZhangDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Sukrut H KarandikarDepartment of Immunology, University of Connecticut School of Medicine, Farmington, CT, 06030, USA.
Rishikesh PandeyConnecticut Children's Innovation Center, University of Connecticut School of Medicine, Farmington, CT, 06032, USA; Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269, USA. Electronic address: rishikesh.pandey@uconn.edu.
Ishan BarmanDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Department of Oncology, Johns Hopkins University, Baltimore, MD, 21287, USA. Electronic address: ibarman@jhu.edu.
Johns Hopkins University · USUniversity of Connecticut · US

Funding

Wide-Field Short-Wave Infrared (SWIR) multiphoton (MP) tissue imagingP41EB015871 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SO, PETER T. · 2012 to 2021
$11.3M
Spectroscopy assisted mechano-chemical phenotype recognition nanoscopeDP2GM128198 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI BARMAN, ISHAN · 2017 to 2017
$2.5M
Determination of functional and molecular biomarkers of treatment resistance with multimodal optical spectroscopyR01CA238025 · NCI · UNIVERSITY OF ARKANSAS AT FAYETTEVILLE · PI RAJARAM, NARASIMHAN · 2019 to 2024
$2.0M
NCI NIH HHS R01 CA238025NIBIB NIH HHS P41 EB015871NIGMS NIH HHS DP2 GM128198
6 · The paper itself

Abstract

Acute lymphoblastic leukemia (ALL) is one of the most common malignancies that account for nearly one-third of all pediatric cancers. The current diagnostic assays are time-consuming, labor-intensive, and require expensive reagents. Here, we report a label-free approach featuring diffraction phase imaging and Raman microscopy that can retrieve both morphological and molecular attributes for label-free optical phenotyping of individual B cells. By investigating leukemia cell lines of early and late stages along with the healthy B cells, we show that phase images can capture subtle morphological differences among the healthy, early, and late stages of leukemic cells. By exploiting its biomolecular specificity, we demonstrate that Raman microscopy is capable of accurately identifying not only different stages of leukemia cells but also individual cell lines at each stage. Overall, our study provides a rationale for employing this hybrid modality to screen leukemia cells using the widefield QPI and using Raman microscopy for accurate differentiation of early and late-stage phenotypes. This contrast-free and rapid diagnostic tool exhibits great promise for clinical diagnosis and staging of leukemia in the near future.

Indexed as

Biosensing TechniquesPrecursor Cell Lymphoblastic Leukemia-LymphomaB-LymphocytesCell LineChildHumansMicroscopyB-ALLLabel-free microscopyLeukemiaPhenotype recognitionQuantitative phase imagingRaman spectroscopyRandom forest classification

Identifiers

PMID34130086
PMCPMC8492164
OpenAlexW3171608433

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.