Evidence map›Paper›PMID 34127068›Full record

ArticleCell & bioscience2021

Electric signals counterbalanced posterior vs anterior PTEN signaling in directed migration of Dictyostelium.

Bing Song, Yu Gu, Wenkai Jiang, Ying Li, Wayne Nishio Ayre, Zhipeng Liu, Tao Yin, Christopher Janetopoulos, Miho Iijima, Peter Devreotes and 1 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Bing SongSchool of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, CF14 4XY, UK. SongB3@cardiff.ac.uk.ORCID http://orcid.org/0000-0001-9356-2333
Yu GuSchool of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, CF14 4XY, UK.
Wenkai JiangSchool of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, CF14 4XY, UK.
Ying LiSchool of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, CF14 4XY, UK.
Wayne Nishio AyreSchool of Dentistry, College of Biomedical and Life Sciences, Cardiff University, Cardiff, CF14 4XY, UK.
Zhipeng LiuChinese Academy of Medical Sciences & Peking Union Medical College Institute of Biomedical Engineering, Tianjin, China.
Tao YinChinese Academy of Medical Sciences & Peking Union Medical College Institute of Biomedical Engineering, Tianjin, China.
Christopher JanetopoulosBioImaging Core Facility, University of Sciences, Philadelphia, PA, 19104, USA.
Miho IijimaSchool of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
Peter DevreotesSchool of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA.
Min ZhaoDepartment of Ophthalmology & Vision Science, UC Davis, School of Medicine, Davis, CA, 95618, USA. minzhao@ucdavis.edu.
Cardiff University · GBChinese Academy of Medical Sciences & Peking Union Medical College · CNJohns Hopkins University · USUniversity of California, Davis · US

Funding

Excitable Networks in Directed Cell MigrationR35GM118177 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Peter N Devreotes · 2016 to 2026
$12.1M
Molecular Generators at Corneal Wounds Produce and regulate the Wound Electrical SignalsR01EY019101 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHAO, MIN · 2010 to 2023
$5.2M
Regulation of Intracellular SignalingR35GM131768 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Miho Iijima · 2019 to 2026
$4.0M
CAMS Initiative for Innovative Medicine 2016-I2M1-004European Research Council 243261National Natural Science Foundation of China 81927806NEI NIH HHS P-30 EY01257NEI NIH HHS R01 EY019101NIGMS NIH HHS R35 GM118177NIGMS NIH HHS R35 GM131768NIH HHS EY019101Royal Society, UK UF051616Wellcome TrustWellcome Trust WT082887MA
6 · The paper itself

Abstract

backgroundCells show directed migration response to electric signals, namely electrotaxis or galvanotaxis. PI3K and PTEN jointly play counterbalancing roles in this event via a bilateral regulation of PIP3 signaling. PI3K has been proved essential in anterior signaling of electrotaxing cells, whilst the role of PTEN remains elusive.

methodsDictyostelium cells with different genetic backgrounds were treated with direct current electric signals to investigate the genetic regulation of electrotaxis.

resultsWe demonstrated that electric signals promoted PTEN phosphatase activity and asymmetrical translocation to the posterior plasma membrane of the electrotaxing cells. Electric stimulation produced a similar but delayed rear redistribution of myosin II, immediately before electrotaxis started. Actin polymerization is required for the asymmetric membrane translocation of PTEN and myosin. PTEN signaling is also responsible for the asymmetric anterior redistribution of PIP3/F-actin, and a biased redistribution of pseudopod protrusion in the forwarding direction of electrotaxing cells.

conclusionsPTEN controls electrotaxis by coordinately regulating asymmetric redistribution of myosin to the posterior, and PIP3/F-actin to the anterior region of the directed migration cells.

Indexed as

Cell migrationElectric fieldElectrotaxisF-actinGalvanotaxisMyosinPH-CracPIP3PTEN

Identifiers

PMID34127068
PMCPMC8201722
OpenAlexW3166471239

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.