Evidence map›Paper›PMID 34127058›Full record

ArticleMicrobiome2021

Roux-en-Y gastric bypass-induced bacterial perturbation contributes to altered host-bacterial co-metabolic phenotype.

Jia V Li, Hutan Ashrafian, Magali Sarafian, Daniel Homola, Laura Rushton, Grace Barker, Paula Momo Cabrera, Matthew R Lewis, Ara Darzi, Edward Lin and 8 more

Open access · goldAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
6.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.

  1. Roles of the gut microbiome in weight management.Nature reviews. Microbiology · 2023
    Pooled it
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  16. Microbiota and gastric cancer: from molecular mechanisms to therapeutic strategies.Frontiers in cellular and infection microbiology · 2025
    Review
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  18. Article
  19. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 6 institutions in 4 countries.

Jia V LiDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Hutan AshrafianDivision of Surgery, Department of Surgery and Cancer, Imperial College London, London, SW7 2AZ, UK.
Magali SarafianDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Daniel HomolaDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Laura RushtonDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Grace BarkerDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Paula Momo CabreraDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Matthew R LewisDivision of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Ara DarziDivision of Surgery, Department of Surgery and Cancer, Imperial College London, London, SW7 2AZ, UK.
Edward LinDivision of General and Gastrointestinal Surgery, Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, 30322, USA.
Nana Adwoa Gletsu-MillerDepartment of Applied Health Science, School of Public Health, Indiana University Bloomington, 1025 E 7th Street, Bloomington, IN, 47405, USA.
Stephen L AtkinRCSI Bahrain, Adiya, Kingdom of Bahrain.
Thozhukat SathyapalanDepartment of Academic Endocrinology, Diabetes and Metabolism, Hull-York Medical School, Hull, UK.
Nigel J GooderhamDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Jeremy K NicholsonCentre for Computational and Systems Medicine, The Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, WA, 6150, Australia.
Julian R MarchesiDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK.
Thanos AthanasiouDivision of Surgery, Department of Surgery and Cancer, Imperial College London, London, SW7 2AZ, UK.
Elaine HolmesDivision of Digestive Disease, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, UK. elaine.holmes@imperial.ac.uk.ORCID 0000-0002-0556-8389
Imperial College London · GBMurdoch University · AUEmory University · USHull York Medical School · GBIndiana University Bloomington · USRoyal College of Surgeons in Ireland - Bahrain · BH

Funding

Plasma Proteome Profiling of Insulin ResistanceR21DK075745 · NIDDK · EMORY UNIVERSITY · PI GLETSU MILLER, NANA · 2007 to 2008
$417k
Department of HealthMedical Research Council MR/L009803/1Medical Research Council MR/P002536/1NIDDK NIH HHS R21 DK075745
6 · The paper itself

Abstract

backgroundBariatric surgery, used to achieve effective weight loss in individuals with severe obesity, modifies the gut microbiota and systemic metabolism in both humans and animal models. The aim of the current study was to understand better the metabolic functions of the altered gut microbiome by conducting deep phenotyping of bariatric surgery patients and bacterial culturing to investigate causality of the metabolic observations.

methodsThree bariatric cohorts (n = 84, n = 14 and n = 9) with patients who had undergone Roux-en-Y gastric bypass (RYGB), sleeve gastrectomy (SG) or laparoscopic gastric banding (LGB), respectively, were enrolled. Metabolic and 16S rRNA bacterial profiles were compared between pre- and post-surgery. Faeces from RYGB patients and bacterial isolates were cultured to experimentally associate the observed metabolic changes in biofluids with the altered gut microbiome.

resultsCompared to SG and LGB, RYGB induced the greatest weight loss and most profound metabolic and bacterial changes. RYGB patients showed increased aromatic amino acids-based host-bacterial co-metabolism, resulting in increased urinary excretion of 4-hydroxyphenylacetate, phenylacetylglutamine, 4-cresyl sulphate and indoxyl sulphate, and increased faecal excretion of tyramine and phenylacetate. Bacterial degradation of choline was increased as evidenced by altered urinary trimethylamine-N-oxide and dimethylamine excretion and faecal concentrations of dimethylamine. RYGB patients' bacteria had a greater capacity to produce tyramine from tyrosine, phenylalanine to phenylacetate and tryptophan to indole and tryptamine, compared to the microbiota from non-surgery, normal weight individuals. 3-Hydroxydicarboxylic acid metabolism and urinary excretion of primary bile acids, serum BCAAs and dimethyl sulfone were also perturbed following bariatric surgery.

conclusionAltered bacterial composition and metabolism contribute to metabolic observations in biofluids of patients following RYGB surgery. The impact of these changes on the functional clinical outcomes requires further investigation. Video abstract.

Indexed as

Gastric BypassObesity, MorbidAnimalsBacteriaHumansPhenotypeRNA, Ribosomal, 16SRNA, Ribosomal, 16SBariatric surgeryBile acidsHost-microbial metabolism, Metabolic profilingMicrobiome

Identifiers

PMID34127058
PMCPMC8201742
OpenAlexW3168240826

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.