Evidence map›Paper›PMID 34125902›Full record

ArticleEndocrinology2021

Physiological Characterization and Transcriptomic Properties of GnRH Neurons Derived From Human Stem Cells.

Kim L Keen, Andrew J Petersen, Alexander G Figueroa, Benjamin I Fordyce, Jaeweon Shin, Rachita Yadav, Serkan Erdin, Robert A Pearce, Michael E Talkowski, Anita Bhattacharyya and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
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  6. Review
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  9. Review
  10. Epigenetics of functional hypothalamic amenorrhea.Frontiers in endocrinology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Kim L KeenWisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA.
Andrew J PetersenWaisman Center, Graduate School, University of Wisconsin, Madison, WI, USA.
Alexander G FigueroaDepartment of Anesthesiology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA.
Benjamin I FordyceWisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA.
Jaeweon ShinCenter for Genomic Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Rachita YadavCenter for Genomic Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Serkan ErdinCenter for Genomic Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Robert A PearceDepartment of Anesthesiology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA.
Michael E TalkowskiCenter for Genomic Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
Anita BhattacharyyaWaisman Center, Graduate School, University of Wisconsin, Madison, WI, USA.
Ei TerasawaWisconsin National Primate Research Center, University of Wisconsin, Madison, WI, USA.ORCID 0000-0003-3995-6643
University of Wisconsin–Madison · USBroad Institute · USHarvard University · US

Funding

WNPRC Supplemental Request for Nonhuman Primate Enclosures to Equip HIV/AIDS-Related Research FacilitiesP51OD011106 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI Dorota A. Grejner-Brzezinska · 2012 to 2026
$150.7M
ZOLEDRONATE PREVENTS BONE LOSS IN OVARIECTOMIZED RHESUS MONKEYSP51RR000167 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI KAUFMAN, PAUL L · 1985 to 2011
$106.7M
University of Wisconsin Institute for Clinical and Translational ResearchUL1TR002373 · NCATS · UNIVERSITY OF WISCONSIN-MADISON · PI ELIZABETH S BURNSIDE, Allan R. Brasier · 2017 to 2026
$75.9M
The Massachusetts General Hospital Harvard Center for Reproductive MedicineP50HD104224 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI TALKOWSKI, MICHAEL E · 2021 to 2025
$7.8M
Waisman Intellectual and Developmental Disabilities Research Center Down Syndrome RegistryU54HD090256 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI MESSING, ALBEE · 2016 to 2020
$6.0M
Molecular mechanisms and genetic drivers of reciprocal genomic disordersR01HD096326 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI TALKOWSKI, MICHAEL E · 2018 to 2022
$3.5M
Role of neuroestradiol in regulation of the GnRH surgeR01HD089495 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI TERASAWA-GRILLEY, EI · 2018 to 2022
$2.1M
Stem Cell-derived GnRH Neurons: Optimization and CharacterizationR21HD092009 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI TERASAWA-GRILLEY, EI · 2017 to 2018
$421k
Role of neuroestrogens in control of GnRH releaseR21HD077447 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI TERASAWA-GRILLEY, EI · 2014 to 2015
$409k
NCATS NIH HHS UL1 TR002373NCRR NIH HHS P51 RR000167NICHD NIH HHS P50 HD104224NICHD NIH HHS R01 HD089495NICHD NIH HHS R01 HD096326NICHD NIH HHS R21 HD077447NICHD NIH HHS R21 HD092009NICHD NIH HHS U54 HD090256NIH HHS P51 OD011106
6 · The paper itself

Abstract

Gonadotropin-releasing hormone (GnRH) neurons in the hypothalamus play a key role in the regulation of reproductive function. In this study, we sought an efficient method for generating GnRH neurons from human embryonic and induced pluripotent stem cells (hESC and hiPSC, respectively). First, we found that exposure of primitive neuroepithelial cells, rather than neuroprogenitor cells, to fibroblast growth factor 8 (FGF8), was more effective in generating GnRH neurons. Second, addition of kisspeptin to FGF8 further increased the efficiency rates of GnRH neurogeneration. Third, we generated a fluorescent marker mCherry labeled human embryonic GnRH cell line (mCh-hESC) using a CRISPR-Cas9 targeting approach. Fourth, we examined physiological characteristics of GnRH (mCh-hESC) neurons: similar to GnRH neurons in vivo, they released the GnRH peptide in a pulsatile manner at ~60 min intervals; GnRH release increased in response to high potassium, kisspeptin, estradiol, and neurokinin B challenges; and injection of depolarizing current induced action potentials. Finally, we characterized developmental changes in transcriptomes of GnRH neurons using hESC, hiPSC, and mCh-hESC. The developmental pattern of transcriptomes was remarkably similar among the 3 cell lines. Collectively, human stem cell-derived GnRH neurons will be an important tool for establishing disease models to understand diseases, such as idiopathic hypothalamic hypogonadism, and testing contraceptive drugs.

Indexed as

Cell DifferentiationCells, CulturedFibroblast Growth Factor 8Gene Expression ProfilingGene Expression Regulation, DevelopmentalGonadotropin-Releasing HormoneHuman Embryonic Stem CellsHumansInduced Pluripotent Stem CellsNeurogenesisNeuronsTranscriptomeFGF8 protein, humanFibroblast Growth Factor 8Gonadotropin-Releasing HormoneGnRHhuman stem cellinduced pluripotent stem cellkisspeptin

Identifiers

PMID34125902
PMCPMC8294693
OpenAlexW3167268671

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.